Sikora Joanna, Cyrankiewicz Michał, Wybranowski Tomasz, Ziomkowska Blanka, Ośmiałowski Borys, Obońska Ewa, Augustyńska Beata, Kruszewski Stefan, Kubica Jacek
Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Medicine, Department of Cardiology and Internal Medicine, Sklodowskiej-Curie Street No. 9, 85-094 Bydgoszcz, PolandbNicolaus Copernicus University, Collegium Medicum in Bydgoszcz.
Nicolaus Copernicus University, Collegium Medicum in Bydgoszcz, Faculty of Pharmacy, Department of Biophysics, Jagiellonska Street No. 13, 85-094 Bydgoszcz, Poland.
J Biomed Opt. 2015 May;20(5):051039. doi: 10.1117/1.JBO.20.5.051039.
The concentration of collagen degradation products (CDPs) may reflect the process of left ventricular remodeling (LVR). The aim of this study was to evaluate the potential diagnostic usefulness of time-resolved fluorescence spectroscopy (TRFS) in assessment of CDPs. The preliminary experiment was designed to establish if CDPs’ characteristics might be visible by mean fluorescence lifetime (FLT) in determined conditions. The in vitro model of CDPs was prepared by conducting the hydrolysis of type III collagen. The FLT of samples was measured by the time-resolved spectrometer Life Spec II with the subnanosecond pulsed 360-nm EPLED diode. The FLTs were obtained by deconvolution analysis of the data using a multiexponential model of fluorescence decay. In order to determine the limit of traceability of CDPs, a comparison of different collagen/plasma ratio in samples was performed. The results of our study showed that the increase of added plasma to hydrolyzed collagen extended the mean FLT. Thus, the diagnosis of LVR based on measurements using TRFS is possible. However, it is important to point out the experiment was preliminary and further investigation in this field of research is crucial.
胶原蛋白降解产物（CDPs）的浓度可能反映左心室重构（LVR）的过程。本研究的目的是评估时间分辨荧光光谱法（TRFS）在评估CDPs方面的潜在诊断价值。初步实验旨在确定在特定条件下，通过平均荧光寿命（FLT）是否可以观察到CDPs的特征。通过对III型胶原蛋白进行水解制备了CDPs的体外模型。使用带有亚纳秒脉冲360纳米EPLED二极管的时间分辨光谱仪Life Spec II测量样品的FLT。通过使用荧光衰减的多指数模型对数据进行去卷积分析获得FLT。为了确定CDPs的可追溯性极限，对样品中不同胶原蛋白/血浆比例进行了比较。我们的研究结果表明，向水解胶原蛋白中添加血浆会延长平均FLT。因此，基于TRFS测量诊断LVR是可能的。然而，需要指出的是，该实验是初步的，在该研究领域进行进一步调查至关重要。
