Li Zhili, Wang Jigui, Yuan Daoli, Wang Shuang, Sun Jiazeng, Yi Bao, Hou Qiang, Mao Yaping, Liu Weiquan
State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.
Virus Genes. 2015 Jun;50(3):434-41. doi: 10.1007/s11262-015-1169-x. Epub 2015 Mar 13.
Canine distemper virus (CDV) and rabies virus (RV) are two important pathogens of the dog. CDV, a member of the morbillivirus genus, has shown promise as an expression vector. The glycoprotein from RV is a main contributor to protective immunity and capable of eliciting the production of virus-neutralizing antibodies. In this study, we recovered an attenuated strain of canine distemper virus and constructed a recombinant virus, rCDV-RV-G, expressing a modified (R333Q) rabies virus glycoprotein (RV-G) of RV Flury strain LEP. RV-G expression by the recombinant viruses was confirmed. Furthermore, G was proved to be incorporated into the surface of CDV particles. While replication of the recombinant virus was slightly reduced compared with the parental CDV, it stably expressed the RV-G over ten serial passages. Inoculation of mice induced specific neutralizing antibodies against both RV-G and CDV. Therefore, the rCDV-RV-G has the potential as a vaccine that may be used to control rabies virus infection in dogs and other animals.
犬瘟热病毒（CDV）和狂犬病病毒（RV）是犬类的两种重要病原体。CDV是麻疹病毒属的成员，已显示出作为表达载体的潜力。RV的糖蛋白是保护性免疫的主要贡献者，能够引发病毒中和抗体的产生。在本研究中，我们获得了一株减毒犬瘟热病毒，并构建了一种重组病毒rCDV-RV-G，其表达RV弗吕里株LEP的修饰（R333Q）狂犬病病毒糖蛋白（RV-G）。证实了重组病毒表达RV-G。此外，证明G被整合到CDV颗粒的表面。虽然重组病毒的复制与亲本CDV相比略有减少，但它在连续十代传代中稳定表达RV-G。接种小鼠可诱导针对RV-G和CDV的特异性中和抗体。因此，rCDV-RV-G有潜力作为一种疫苗，可用于控制犬类和其他动物的狂犬病病毒感染。
