HLA-I类基因限制性片段长度多态性与中国强直性脊柱炎患者的相关性

Association of RFLP of HLA class I genes with Chinese ankylosing spondylitis patients.

作者信息

Lin X Y, Wang Y, Sun J, Wang F Q, Ni L Q, Chang L Y, Chen S S

机构信息

Department of Biochemistry, Shanghai Second Medical University, China.

出版信息

Tissue Antigens. 1989 Nov;34(5):279-83. doi: 10.1111/j.1399-0039.1989.tb01743.x.

DOI:10.1111/j.1399-0039.1989.tb01743.x

PMID:2576476

Abstract

By serum typing, it is indicated that HLA B27 may be associated with the susceptibility to ankylosing spondylitis (AS). We analyzed DNA restriction fragment length polymorphism (RFLP) in 28 Chinese AS patients and 99 healthy controls, using a 1.4 Kb HLA class I cDNA probe. The results showed that the frequencies of the 8.1 Kb EcoRI, 5.2 Kb EcoRI and 21.9 Kb XbaI fragments were found to be significantly increased in affected patients (P less than 0.0001, P = 0.0015, respectively), but that of 19.2 Kb XbaI fragment was decreased (P = 0.0021). The data suggest that AS may be a polygenic disease; furthermore, B27 and 8.1 Kb EcoRI band may be two different factors responsible for the susceptibility or just in linkage disequilibrium with the susceptible gene(s).

摘要

血清分型表明，HLA B27可能与强直性脊柱炎（AS）的易感性有关。我们使用1.4 Kb HLA I类cDNA探针分析了28例中国AS患者和99例健康对照者的DNA限制性片段长度多态性（RFLP）。结果显示，8.1 Kb EcoRI、5.2 Kb EcoRI和21.9 Kb XbaI片段的频率在患病患者中显著增加（分别为P小于0.0001，P = 0.0015），但19.2 Kb XbaI片段的频率降低（P = 0.0021）。数据表明，AS可能是一种多基因疾病；此外，B27和8.1 Kb EcoRI条带可能是导致易感性的两个不同因素，或者只是与易感基因处于连锁不平衡状态。