Hornemann Andrea, Eichert Diane, Flemig Sabine, Ulm Gerhard, Beckhoff Burkhard
Physikalisch-Technische Bundesanstalt, Abbestr. 2-12, 10587 Berlin, Germany.
Phys Chem Chem Phys. 2015 Apr 14;17(14):9471-9. doi: 10.1039/c4cp05944a. Epub 2015 Mar 13.
The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore-antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.
在高复杂性生物诊断分析中，对技术进步的需求既需要基础研究，也需要在纳米级分析识别元件方面进行构建工作，这些元件能够提供独特的选择性和设计增强的灵敏度特性。纳米颗粒诱导的灵敏度增强及其与多重检测能力相关的应用，表面增强红外吸收（SEIRA）分析形式非常适合这些目的。已经研究了多种荧光团 - 抗体缀合物化学吸附到低成本金纳米颗粒SEIRA底物上的潜力，考察了它们的光谱可区分性。这些新型生物标记物提供了分子SEIRA指纹图谱，已通过单变量和多变量分析工具成功分析，以区分它们的多重检测能力。我们表明，通过SEIRA指纹图谱进行的这种强大的光谱编码为生物诊断中的快速、可靠和多重高端筛选开辟了新机会。
