平肝潜阳方对肝阳上亢证自发性高血压大鼠下丘脑Tpx II、HSP27及ANXA1表达的影响

[Effect of Pinggan Qianyang recipe on the expression of Tpx II HSP27 and ANXA1 in the hypothalamus of spontaneously hypertensive rats with hyperactivity of liver-YANG syndrome].

作者信息

Zhong Guangwei, Xiang Lingli, Hu Jianjun, Yin Yaohui, Chen Qiong, Fang Xia

机构信息

Institute of Integrated Traditional Chinese and Western Medicine; Department of Geriatrics, Xiangya Hospital, Central South University, Changsha 410008, China.

出版信息

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2015 Feb;40(2):136-43. doi: 10.11817/j.issn.1672-7347.2015.02.004.

DOI:10.11817/j.issn.1672-7347.2015.02.004

PMID:25769321

Abstract

OBJECTIVE

To investigate the effect Pinggan Qianyang recipe on expression of Tpx, HSP27 and ANXA1 in the hypothalamus of spontaneously hypertensive rats (SHRs) with the hyperactivity of liver-YANG syndrome.

METHODS

A total of 30 SHRs were subjected to administration of Aconiti Praeparatae Decoction to establish the model of SHR with liver-YANG hyperactivity first, then they were randomly divided into three groups: the control group, the model group and the treatment group (n=10 per group). A total of 10 SD rats were served as the normal group. The rats in control group and treatment group were given Enalapril plus Pinggan Qianyang recipe for four weeks. The change of behavior and blood pressure of rats were monitored. RT-PCR and Western-blot were performed to detect the expression of Tpx II, HSP27 and ANXA1 mRNA and protein in the hypothalamus, respectively.

RESULTS

Compared with the normal SD rats, the heart rate, blood pressure and grade of irritability were significantly increased while rotation endurance time was dramatically reduced in the SHR model with liver-YANG hyperactivity (P<0.01), these changes were reversed by the application of Enalapril plus Pinggan Qianyang recipe. Compared with the normal SD rats, the protein and mRNA expression of Tpx II and ANXA1 in the model group were significantly upregulated (P<0.01) while the HSP27 was significantly downregulated (P<0.01). Compared with the model group, the protein and mRNA expression of Tpx II and ANXA1 in the control group or treatment group were significantly decreased (P<0.05 or P<0.01) while HSP27 was significantly increased (P<0.05 or P<0.01). Compared with the control group, the expression of Tpx II and ANXA1 protein in treatment group were significantly reduced (P<0.05 or P<0.01).

CONCLUSION

Pinggan Qianyang recipe can improve the blood pressure and behavior in SHRs with hyperactivity of Liver-YANG syndrome, which might be related to the regulation of Tpx, HSP27 and ANXA1 expression in hypothalamuses.

摘要

目的

探讨平肝潜阳方对肝阳上亢证自发性高血压大鼠（SHRs）下丘脑Tpx、HSP27和ANXA1表达的影响。

方法

将30只SHRs先用附子汤灌胃建立肝阳上亢证SHR模型，然后随机分为三组：对照组、模型组和治疗组（每组10只）。另取10只SD大鼠作为正常组。对照组和治疗组大鼠给予依那普利加平肝潜阳方灌胃四周。监测大鼠行为和血压变化。分别采用RT-PCR和Western-blot法检测下丘脑Tpx II、HSP27和ANXA1 mRNA及蛋白表达。

结果

与正常SD大鼠相比，肝阳上亢证SHR模型大鼠心率、血压和易怒等级显著升高，而旋转耐力时间显著缩短（P<0.01），应用依那普利加平肝潜阳方后这些变化得到逆转。与正常SD大鼠相比，模型组Tpx II和ANXA1蛋白及mRNA表达显著上调（P<0.01），而HSP27显著下调（P<0.01）。与模型组相比，对照组或治疗组Tpx II和ANXA1蛋白及mRNA表达显著降低（P<0.05或P<0.01），而HSP27显著升高（P<0.05或P<0.01）。与对照组相比，治疗组Tpx II和ANXA1蛋白表达显著降低（P<0.05或P<0.01）。