Park Changhoon, Choi Kyuhyung, Jeong Jiwoon, Chae Chanhee
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 151-742, Republic of Korea.
Vet Microbiol. 2015 May 15;177(1-2):87-94. doi: 10.1016/j.vetmic.2015.02.020. Epub 2015 Feb 26.
The objective of the present study was to determine the cross-protection of a new type 2 porcine reproductive and respiratory syndrome virus (PRRSV) modified live vaccine against heterologous type 1 PRRSV challenge in growing pigs. The mean rectal temperature and respiratory score was significantly (P<0.05) lower in vaccinated challenged pigs than in unvaccinated challenged pigs. Vaccination of pigs with type 2 PRRSV reduced the levels of type 1 PRRSV viremia after challenge with type 1 PRRSV. Vaccinated challenged pigs had significantly (P<0.05) higher frequency of interferon-γ secreting cells and lower levels of interleukin-10 compared to unvaccinated challenged pigs. Vaccination of pigs with the type 2 PRRSV effectively reduced the macroscopic and microscopic lung lesion and the type 1 PRRSV antigens within lung lesions in vaccinated challenged pigs. This study demonstrates partial cross-protection of a new type 2 PRRSV modified live vaccine against heterologous type 1 PRRSV challenge in growing pigs.
本研究的目的是确定一种新型2型猪繁殖与呼吸综合征病毒（PRRSV）活疫苗对生长猪中异源1型PRRSV攻击的交叉保护作用。与未接种疫苗而受到攻击的猪相比，接种疫苗后受到攻击的猪的平均直肠温度和呼吸评分显著更低（P<0.05）。用2型PRRSV给猪接种疫苗可降低1型PRRSV攻击后1型PRRSV病毒血症水平。与未接种疫苗而受到攻击的猪相比，接种疫苗后受到攻击的猪的γ干扰素分泌细胞频率显著更高（P<0.05），白细胞介素-10水平更低。用2型PRRSV给猪接种疫苗可有效减少接种疫苗后受到攻击的猪的宏观和微观肺部病变以及肺部病变内的1型PRRSV抗原。本研究证明了一种新型2型PRRSV活疫苗对生长猪中异源1型PRRSV攻击具有部分交叉保护作用。
