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在哺乳动物培养细胞的细胞分裂过程中，ZIP激酶介导的肌球蛋白II调节轻链磷酸化作用负责分裂沟的内陷。

Phosphorylation of myosin II regulatory light chain by ZIP kinase is responsible for cleavage furrow ingression during cell division in mammalian cultured cells.

作者信息

Hosoba Kosuke, Komatsu Satoshi, Ikebe Mitsuo, Kotani Manato, Wenqin Xiao, Tachibana Taro, Hosoya Hiroshi, Hamao Kozue

机构信息

Department of Biological Science, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan.

Department of Cellular and Molecular Biology, University of Texas Health Northeast, Tyler, TX 75708, USA.

出版信息

Biochem Biophys Res Commun. 2015 Apr 17;459(4):686-91. doi: 10.1016/j.bbrc.2015.03.005. Epub 2015 Mar 11.

DOI:10.1016/j.bbrc.2015.03.005
PMID:25769953
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4402231/
Abstract

Zipper-interacting protein kinase (ZIPK) is known to regulate several functions such as apoptosis, smooth muscle contraction, and cell migration. While exogenously expressed GFP-ZIPK localizes to the cleavage furrow, role of ZIPK in cytokinesis is obscure. Here, we show that ZIPK is a major MRLC kinase during mitosis. Moreover, ZIPK siRNA-mediated knockdown causes delay of cytokinesis. The delay in cytokinesis of ZIPK-knockdown cells was rescued by the exogenous diphosphorylation-mimicking MRLC mutant. Taken together, these findings suggest that ZIPK plays a role in the progression and completion of cytokinesis through MRLC phosphorylation.

摘要

已知拉链相互作用蛋白激酶（ZIPK）可调节多种功能，如细胞凋亡、平滑肌收缩和细胞迁移。虽然外源表达的绿色荧光蛋白-ZIPK定位于分裂沟，但ZIPK在胞质分裂中的作用尚不清楚。在这里，我们表明ZIPK是有丝分裂期间的主要肌球蛋白轻链激酶（MRLC激酶）。此外，ZIPK siRNA介导的敲低会导致胞质分裂延迟。外源表达的模拟双磷酸化的MRLC突变体挽救了ZIPK敲低细胞的胞质分裂延迟。综上所述，这些发现表明ZIPK通过MRLC磷酸化在胞质分裂的进程和完成中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/840231bdbef9/nihms671502f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/4b4fae5de8a2/nihms671502f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/b4de0ff2b79b/nihms671502f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/7cd38a8eb3b3/nihms671502f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/840231bdbef9/nihms671502f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/4b4fae5de8a2/nihms671502f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/b4de0ff2b79b/nihms671502f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/7cd38a8eb3b3/nihms671502f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e751/4402231/840231bdbef9/nihms671502f4.jpg

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