从细菌培养基中液-液萃取和液相色谱-质谱联用检测姜黄素类化合物
Liquid-liquid extraction and liquid chromatography-mass spectrometry detection of curcuminoids from bacterial culture medium.
作者信息
Tan Suryani, Rupasinghe Thusitha W T, Tull Dedreia L, Augustin Mary Ann, Gras Sally L
机构信息
CSIRO Food, Nutrition and Bioproducts Flagship, 671 Sneydes Road, Werribee, Vic 3030, Australia; Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Vic 3010, Australia; Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia.
Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, Vic 3010, Australia; Metabolomics Australia, The University of Melbourne, Parkville, Vic 3010, Australia.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Apr 15;988:116-20. doi: 10.1016/j.jchromb.2015.02.024. Epub 2015 Feb 24.
Liquid chromatography-mass spectrometry (LC-MS) has been used to detect polyphenolic curcuminoids found in turmeric but studies of metabolism by bacterial and mammalian cells in vitro are compromised by poor recovery from the culture medium. We report a liquid-liquid extraction procedure with ethyl acetate and use LC-MS to quantify extracted curcuminoids. Ethyl acetate allows recoveries of ∼ 80-86% of curcuminoids from the bacterial growth medium, bacterial cell lysate and combined bacterial cell and growth medium matrices; a clear improvement over acetonitrile where recoveries were ∼ 25-66%. This optimised method will enable studies of curcuminoid metabolism and may be applicable to other hydrophobic polyphenolic compounds.
液相色谱-质谱联用（LC-MS）已被用于检测姜黄中的多酚类姜黄素，但体外细菌和哺乳动物细胞的代谢研究因从培养基中的回收率低而受到影响。我们报告了一种用乙酸乙酯进行液液萃取的方法，并使用LC-MS对萃取的姜黄素进行定量。乙酸乙酯能从细菌生长培养基、细菌细胞裂解物以及细菌细胞与生长培养基的混合基质中回收约80-86%的姜黄素；与回收率约为25-66%的乙腈相比有明显改善。这种优化后的方法将有助于姜黄素代谢的研究，并且可能适用于其他疏水性多酚化合物。
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