Jiang Hongliang, Timmermann Barbara N, Gang David R
Arizona Center for Phytomedicine Research, College of Pharmacy, University of Arizona, Tucson, 85721, USA.
J Chromatogr A. 2006 Apr 7;1111(1):21-31. doi: 10.1016/j.chroma.2006.01.103. Epub 2006 Feb 21.
LC-ESI-MS/MS coupled to DAD analysis was used as an on-line tool for identification of diarylheptanoids in fresh turmeric rhizome extracts. Based on their mass spectra, from both negative and positive mode LC-ESI-MS/MS analysis, and supported by their DAD spectra, 19 diarylheptanoids were identified. Among these 19 compounds, curcumin, demethoxycurcumin, and bisdemethoxycurcumin were identified by comparing their chromatographic and spectral data with those of authentic standard compounds. The other diarylheptanoid compounds were identified or tentatively identified based on comparison to the three curcuminoids and each other. Twelve of the identified diarylheptanoids have not been previously reported from turmeric and six of these are new compounds.
液相色谱-电喷雾串联质谱联用二极管阵列检测（LC-ESI-MS/MS-DAD）分析被用作在线工具，用于鉴定新鲜姜黄根茎提取物中的二芳基庚烷类化合物。基于其质谱（来自负离子和正离子模式的LC-ESI-MS/MS分析），并得到二极管阵列检测（DAD）光谱的支持，鉴定出19种二芳基庚烷类化合物。在这19种化合物中，通过将其色谱和光谱数据与标准品的色谱和光谱数据进行比较，鉴定出了姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。其他二芳基庚烷类化合物则是通过与三种姜黄素类化合物相互比较而被鉴定或初步鉴定。已鉴定出的19种二芳基庚烷类化合物中有12种此前尚未在姜黄中报道过，其中6种是新化合物。
