UFZ-Helmholtz Center for Environmental Research, Department of Environmental Engineering, Permoserstr. 15, D-04318 Leipzig, Germany.
UFZ-Helmholtz Center for Environmental Research, Department of Environmental Biotechnology, Permoserstr. 15, D-04318 Leipzig, Germany.
Sci Total Environ. 2015 Jun 15;518-519:371-7. doi: 10.1016/j.scitotenv.2015.02.099. Epub 2015 Mar 13.
Tetramethylammonium hydroxide-assisted thermochemolysis performed in an off-line mode proved a useful tool in determining organic compounds in the effluent from laboratory-scale phytoremediation systems. Studies were performed with artificial wastewaters contaminated with xylenols and densely rooted Juncus effuses plants. Analytes in these molecular-level based studies included xylenol substrates, an array of stable intermediates such as low molecular weight carboxylic acids and oxidative coupling products (tetramethyl biphenyldiols, tetramethyl diphenylether monools), diagnostic fatty acid biomarkers, as well as lignin-, carbohydrate-, and protein-based phenols and carboxylic acids. Lignin-based breakdown products belonged to p-hydroxyphenyl- and guaiacyl-units, with lower abundance of syringyl units and the dominance of acids over phenols. Monomeric lignin-, protein- and carbohydrate-based breakdown products could not be detected in the non-treated lyophilized effluent. The formation of diketopiperazines pointed to soluble peptides and proteins. The procedure described herein can easily be applied in every modern laboratory to characterize underlying processes in phytoremediation.
四甲基氢氧化铵辅助的离线热化学解在确定实验室规模植物修复系统流出物中的有机化合物方面证明是一种有用的工具。研究采用人工废水进行，这些废水中含有二甲苯酚和密集扎根的灯心草属植物。这些基于分子水平的研究中的分析物包括二甲苯酚底物、一系列稳定的中间体，如低分子量羧酸和氧化偶联产物（四甲基联苯二醇、四甲基二苯醚单醇）、诊断脂肪酸生物标志物以及木质素、碳水化合物和蛋白质基酚和羧酸。木质素基分解产物属于对羟基苯基和愈创木基单元，丁香基单元的丰度较低，酸的丰度高于酚。未处理的冻干流出物中未检测到单体木质素、蛋白质和碳水化合物基分解产物。二酮哌嗪的形成表明存在可溶性肽和蛋白质。本文所述的方法可以很容易地应用于每个现代实验室，以表征植物修复中的潜在过程。
