在非典型韦荣球菌中建立一种可反向选择的无标记诱变系统。
Establishment of a counter-selectable markerless mutagenesis system in Veillonella atypica.
作者信息
Zhou Peng, Li Xiaoli, Qi Fengxia
机构信息
Department of Oral Biology, College of Dentistry, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
Department of Oral Biology, College of Dentistry, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, United States.
出版信息
J Microbiol Methods. 2015 May;112:70-2. doi: 10.1016/j.mimet.2015.03.010. Epub 2015 Mar 12.
Abstract
Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella.
摘要
以替代的σ因子ecf3为靶点，我们成功地在韦荣氏菌属中建立了首个无标记诱变系统。该系统将成为韦荣氏菌属中用于多基因诱变以进行基因功能分析以及基因调控研究的宝贵工具。
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