Sun H-Z, Yu K-H, Ai H-B
College of Life Science, Qi Lu Normal University, Jinan, China.
Neurogastroenterol Motil. 2015 May;27(5):618-26. doi: 10.1111/nmo.12530. Epub 2015 Mar 14.
Hydrogen sulfide (H2 S) is a gaseous messenger and serves as an important neuromodulator in the central nervous system. This study aimed to clarify the role of H2 S within the dorsal motor nucleus of the vagus (DMV) in the control of gastric function in rats.
Cystathionine β-synthetase (CBS) is an important generator of endogenous H2 S in the brain. We investigated the distribution of CBS in the DMV using immunohistochemical method, and the effects of H2 S on gastric motility and on gastric acid secretion.
CBS-immunoreactive (IR) neurons were detected in the rostral, intermediate and caudal DMV, with the highest number of CBS-IR neurons in the caudal DMV, and the lowest in the intermediate DMV. We also found that microinjection of the exogenous H2 S donor NaHS (0.04 and 0.08 mol/L; 0.1 μL; n = 6; p < 0.05) into the DMV significantly inhibited gastric motility with a dose-dependent trend, and promoted gastric acid secretion in Wistar rats. Microinjection of the same volume of physiological saline (PS; 0.1 μL, n = 6, p > 0.05) at the same location did not noticeably change gastric motility and acid secretion.
CONCLUSIONS & INFERENCES: The data from these experiments suggest that the CBS that produces H2 S is present in the DMV, and microinjection of NaHS into the DMV inhibited gastric motility and enhanced gastric acid secretion in rats.
硫化氢（H₂S）是一种气体信使，在中枢神经系统中作为重要的神经调节剂发挥作用。本研究旨在阐明H₂S在大鼠迷走神经背核（DMV）中对胃功能控制的作用。
胱硫醚β-合成酶（CBS）是脑内内源性H₂S的重要生成酶。我们采用免疫组化方法研究了CBS在DMV中的分布，以及H₂S对胃动力和胃酸分泌的影响。
在DMV的头端、中间段和尾端均检测到CBS免疫反应性（IR）神经元，其中尾端DMV中CBS-IR神经元数量最多，中间段DMV中最少。我们还发现，向Wistar大鼠的DMV微量注射外源性H₂S供体硫氢化钠（NaHS，0.0ⱼ4和0.08 mol/L；0.1 μL；n = 6；p < 0.05）可显著抑制胃动力，呈剂量依赖性趋势，并促进胃酸分泌。在同一位置微量注射相同体积的生理盐水（PS，0.1 μL，n = 6，p > 0.05）对胃动力和胃酸分泌无明显影响。
这些实验数据表明，产生H₂S的CBS存在于DMV中，向DMV微量注射NaHS可抑制大鼠胃动力并增强胃酸分泌。
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