Sun Hongzhao, Ding Haikun, Shi Yuan, Li Chenyu, Jin Haoran, Yang Xiaoyue, Chen Zhaosong, Tian Pengpeng, Zhu Jianping, Sun Haiji
School of Life Sciences, Qilu Normal University, Jinan, China.
Key Laboratory of Animal Resistance, School of Life Sciences, Shandong Normal University, Jinan, China.
Front Physiol. 2020 Sep 11;11:545184. doi: 10.3389/fphys.2020.545184. eCollection 2020.
Hydrogen sulfide (HS) is a neuromodulator in the central nervous system. However, the physiological role of HS in the nucleus ambiguus (NA) has rarely been reported. This research aimed to elucidate the role of HS in the regulation of gastrointestinal motility in rats. Male Wistar rats were randomly assigned to sodium hydrosulfide (NaHS; 4 and 8 nmol) groups, physiological saline (PS) group, capsazepine (10 pmol) + NaHS (4 nmol) group, L703606 (4 nmol) + NaHS (4 nmol) group, and pyrrolidine dithiocarbamate (PDTC, 4 nmol) + NaHS (4 nmol) group. Gastrointestinal motility curves before and after the injection were recorded using a latex balloon attached with a pressure transducer, which was introduced into the pylorus through gastric fundus. The results demonstrated that NaHS (4 and 8 nmol), an exogenous HS donor, remarkably suppressed gastrointestinal motility in the NA of rats ( < 0.01). The suppressive effect of NaHS on gastrointestinal motility could be prevented by capsazepine, a transient receptor potential vanilloid 1 (TRPV1) antagonist, and PDTC, a NF-κB inhibitor. However, the same amount of PS did not induce significant changes in gastrointestinal motility ( > 0.05). Our findings indicate that NaHS within the NA can remarkably suppress gastrointestinal motility in rats, possibly through TRPV1 channels and NF-κB-dependent mechanism.
硫化氢（HS）是中枢神经系统中的一种神经调节剂。然而，HS在疑核（NA）中的生理作用鲜有报道。本研究旨在阐明HS在调节大鼠胃肠动力中的作用。将雄性Wistar大鼠随机分为氢硫化钠（NaHS；4和8 nmol）组、生理盐水（PS）组、辣椒素受体拮抗剂（10 pmol）+ NaHS（4 nmol）组、L703606（4 nmol）+ NaHS（4 nmol）组以及吡咯烷二硫代氨基甲酸盐（PDTC，4 nmol）+ NaHS（4 nmol）组。使用连接压力传感器的乳胶气球记录注射前后的胃肠动力曲线，该乳胶气球通过胃底插入幽门。结果表明，外源性HS供体NaHS（4和8 nmol）显著抑制大鼠NA中的胃肠动力（<0.01）。辣椒素受体拮抗剂辣椒素和NF-κB抑制剂PDTC可阻止NaHS对胃肠动力的抑制作用。然而，等量的PS并未引起胃肠动力的显著变化（>0.05）。我们的研究结果表明，NA内的NaHS可能通过辣椒素受体1（TRPV1）通道和NF-κB依赖机制显著抑制大鼠的胃肠动力。
