Son Sang Uk, Garrell Robin L
Department of Chemistry and Biochemistry and California NanoSystems Institute, University of California Los Angeles, CA 90095-1569, USA.
Lab Chip. 2009 Aug 21;9(16):2398-401. doi: 10.1039/b906257b. Epub 2009 Jun 5.
We demonstrate the first programmed transport of live yeast (Saccharomyces cerevisiae) and a zebrafish embryo (Danio rerio) within droplets in a two-plate digital microfluidic device. The yeast remained viable after transport, and the actuated droplets left no yeast behind. A zebrafish embryo transported 2 hours after fertilization developed normally and hatched. Dechorionation was demonstrated by mixing a droplet of digestive reagent droplet with a droplet containing the embryo. These results demonstrate the potential for using a droplet microfluidic device as an alternative to microwell plates for yeast and zebrafish assays.
我们展示了在双板数字微流控装置中，活酵母（酿酒酵母）和斑马鱼胚胎（斑马鱼）在液滴内的首次程序化运输。运输后酵母仍保持活力，且被驱动的液滴没有留下任何酵母。受精后2小时运输的斑马鱼胚胎正常发育并孵化。通过将一滴消化试剂液滴与含有胚胎的液滴混合，证明了去绒毛膜的过程。这些结果表明，使用液滴微流控装置作为微孔板用于酵母和斑马鱼实验的替代方案具有潜力。
