Xue Qian, Xing Weiwei, Liu Yongxin, Gong Liang
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Oct;28(19):1483-6.
To observe the effect of tympanic injection of dexamethasone in the guinea pig endolymphatic hydrops and the change CFTR expression, to explore the effect of glucocorticoid treatment endolymphatic and its possible mechanism.
Thirty guinea pigs were divided into three groups: hormone group, water group, control group. The animals(hormone group, water group) in study were injected DDAVP 4 μg/kg in the first 7 d, and will increase to 6 μg/kg in the second 3 d. The control group was given normal saline, continuous 10 d. After twelfth days, the hormone group transtympanic injection of dexamethasone (5 mg/ml, 0.5 ml), and water group, control group transtympanic given normal saline (0.5 ml), continuous injection 5 d. Using immuno- histochemistry and Western blot to detect the cystic fibrosis transmembrane conductance regulator cochlear factor (CFTR) expression.
The water group ABR thresholds was significantly higher than that before the experiment (P < 0.01), and the water group was significantly higher than the rest of the groups (P < 0.01); Hormone group compared with the control group increased threshold value (P < 0.05). The control group had no endolym- phatic hydrops, the water group showed varying degrees of endolymphatic hydrops, cochlear duct and vestibular plus cochlear duct area ratio compared with the control group, hormone group was significantly higher (P < 0.01). hormone group area ratio was higher than the control group (P < 0.05). CFTR was primarily expressed in the stria vascularis, Corti's, spiral ligament, basilar membrane, cochlear ganglion,etc . The expression of CFTR in the water group was increased than that in the control group, and the hormone group (P < 0.01); the expression of hormone group increased compared with the control group (P < 0.05).
Tympanic injection of dexa- methasone can alleviate the desmopressin acetatein guinea pigs caused by membranous labyrinth, and the improve- ment of the hearing; Tympanic injection of dexamethasone can make the endolymphatic hydrops cochlea of guinea pigs decreased CFTR expression, indicating that the expression and possible mechanisms of CFTR intratympanic steroids reduce endolymphatic hydrops changes.
观察鼓室内注射地塞米松对豚鼠膜迷路积水及囊性纤维化跨膜传导调节因子（CFTR）表达变化的影响，探讨糖皮质激素治疗膜迷路积水的作用及其可能机制。
将30只豚鼠分为激素组、水组、对照组。研究中的动物（激素组、水组）在第1个7天腹腔注射去氨加压素4μg/kg，后3天增加至6μg/kg。对照组给予生理盐水，共10天。第12天后，激素组鼓室内注射地塞米松（5mg/ml，0.5ml），水组、对照组鼓室内给予生理盐水（0.5ml），连续注射5天。采用免疫组织化学和蛋白质印迹法检测耳蜗囊性纤维化跨膜传导调节因子（CFTR）表达。
水组听性脑干反应（ABR）阈值较实验前显著升高（P<0.01），且水组显著高于其他组（P<0.01）；激素组与对照组相比阈值升高（P<0.05）。对照组无膜迷路积水，水组呈现不同程度的膜迷路积水，水组蜗管及前庭加蜗管面积比与对照组、激素组相比显著升高（P<0.01）。激素组面积比高于对照组（P<0.05）。CFTR主要表达于血管纹、柯蒂器、螺旋韧带、基底膜、螺旋神经节等。水组CFTR表达高于对照组、激素组（P<0.01）；激素组表达较对照组升高（P<0.05）。
鼓室内注射地塞米松可减轻醋酸去氨加压素所致豚鼠膜迷路积水，并改善听力；鼓室内注射地塞米松可使豚鼠耳蜗膜迷路积水CFTR表达降低，提示CFTR表达变化可能是鼓室内注射类固醇激素减轻膜迷路积水的机制。
