Jiang Liyuan, Wang Canjun, Ni Fangying, Chen Huade
Zhongguo Zhen Jiu. 2015 Jun;35(6):579-84.
To observe the effects of electroacupuncture (EA) on cochlea morphology and expression of aquaporin 1 (AQP1) in guinea pigs with endolymphatic hydrops, so as to explore the possible mechanism of EA on endolymphatic hydrops.
Forty guinea pigs were randomly divided into a blank group, a model group, a medication group and an EA group, 10 guinea pigs in each one. Model of endolymphatic hydrops was established by using intraperitoneal injection of aldosterone. Guinea pigs in the blank group and model group were treated with identical immobilization as EA group but no treatment was given; guinea pigs in the medication group were treated with intragastric administration of hydrochlorothiazide at a dose of 5 mg/kg, once a day for consecutive 10 days; guinea pigs in the EA group were treated with' EA at "Baihui" (GV 20) and "Tinggong"(SI 19), once a day for consecutive 10 days. The serum ionic concentration in each group was tested by turbidimetric method; hematoxylin-eosin staining was used to measure the severity of cochlea hydrops; immunohistochemical method was used to observe the expression of AQP1 in the cochlea.
(1) There was no endolymphatic hydrops in the blank group, moderate-severe endolymphatic hydrops in the model group and slight endolymphatic hydrops in the EA group and medication group. (2) The concentration of K+ and Ca2+ in the EA group was higher than that in the model group and medication group (all P<0. 01); the concentration of Na+ was lower than that in the model group (P< 0. 01) but higher than that in the medication group (P<0. 01); the concentration of Cl- was higher than that in the medication group (P<0. 01), but not significantly different from the model group (P>0. 05). (3) The ratio of expression area of AQP1 in the model group was lower than that in the blank group (P<0. 01); the ratio of expression area of AQP1 in the EA group was higher than that in the model group (P<0. 01), and lower than that in the medication group without significant difference (P>0. 05).
EA could relieve the endolymphatic hydrops in guinea pigs; the mechanism is likely to be related with up-regulating the expression of AQP1 in cochlea and ion concentration might be an important factor involved.
观察电针（EA）对豚鼠内淋巴积水模型耳蜗形态及水通道蛋白1（AQP1）表达的影响，探讨电针对内淋巴积水的作用机制。
40只豚鼠随机分为空白组、模型组、药物组和电针组，每组10只。采用腹腔注射醛固酮法制备内淋巴积水模型。空白组和模型组豚鼠给予与电针组相同的固定处理，但不进行治疗；药物组豚鼠给予氢氯噻嗪5 mg/kg灌胃，每天1次，连续10天；电针组豚鼠于“百会”（GV 20）和“听宫”（SI 19）行电针治疗，每天1次，连续10天。采用比浊法检测各组血清离子浓度；苏木精-伊红染色观察耳蜗积水程度；免疫组化法观察耳蜗AQP1的表达。
（1）空白组无内淋巴积水，模型组为中重度内淋巴积水，电针组和药物组为轻度内淋巴积水。（2）电针组K+、Ca2+浓度高于模型组和药物组（均P<0.01）；Na+浓度低于模型组（P<0.01）但高于药物组（P<0.01）；Cl-浓度高于药物组（P<0.01），与模型组比较差异无统计学意义（P>0.05）。（3）模型组AQP1表达面积比例低于空白组（P<0.01）；电针组AQP1表达面积比例高于模型组（P<0.01），低于药物组但差异无统计学意义（P>0.05）。
电针可减轻豚鼠内淋巴积水；其机制可能与上调耳蜗AQP1表达有关，离子浓度可能是其中的重要因素。
