BCL6表达与B淋巴细胞白血病中的t(1;19)易位相关。

BCL6 expression correlates with the t(1;19) translocation in B-lymphoblastic leukemia.

作者信息

Deucher Anne M, Qi Zhongxia, Yu Jingwei, George Tracy I, Etzell Joan E

机构信息

From the University of California, San Francisco, and

From the University of California, San Francisco, and.

出版信息

Am J Clin Pathol. 2015 Apr;143(4):547-57. doi: 10.1309/AJCPO4U4VYAAOTEL.

DOI:10.1309/AJCPO4U4VYAAOTEL

PMID:25780007

Abstract

OBJECTIVES

Study to date suggests that BCL6 protein expression in B-cell neoplasia predominates in germinal center-derived tumors, but less is known regarding its expression in B-lymphoblastic leukemia. Therefore, we designed a comprehensive study of BCL6 expression in B-lymphoblastic leukemia.

METHODS

BCL6, LMO, and HGAL protein expression in B-lymphoblastic leukemia was investigated using immunohistochemical staining of paraffin-embedded bone marrow specimens. Cryptic TCF3(E2A)-PBX1 rearrangements were investigated using interphase fluorescence in situ hybridization.

RESULTS

Six (12%) of 52 B-lymphoblastic leukemias demonstrated BCL6 protein expression, with B-cell lymphoblastic leukemias containing a t(1;19) translocation demonstrating the strongest staining (three of three). Additional t(1;19) cases beyond the screening study showed similar results. Public microarray expression database mining showed that BCL6 messenger RNA expression levels in B-lymphoblastic leukemia correlated with the protein expression findings. Finally, other markers of B-cell development correlated with BCL6 expression in t(1;19) B-lymphoblastic leukemia cases, with LMO2 and HGAL proteins expressed in six (67%) of nine and eight (89%) of nine cases, respectively.

CONCLUSIONS

BCL6 expression is present in a subset of B-lymphoblastic leukemias, especially in cases containing the 1;19 translocation. Investigation for TCF3(E2A)-PBX1 rearrangements may be useful in BCL6-positive B-lymphoblastic leukemia.

摘要

目的

迄今为止的研究表明，B细胞肿瘤中的BCL6蛋白表达在生发中心来源的肿瘤中占主导，但对于其在B淋巴细胞白血病中的表达了解较少。因此，我们设计了一项关于BCL6在B淋巴细胞白血病中表达的全面研究。

方法

使用石蜡包埋骨髓标本的免疫组织化学染色研究BCL6、LMO和HGAL蛋白在B淋巴细胞白血病中的表达。使用间期荧光原位杂交研究隐匿性TCF3（E2A）-PBX1重排。

结果

52例B淋巴细胞白血病中有6例（12%）表现出BCL6蛋白表达，含有t（1；19）易位的B细胞淋巴细胞白血病显示出最强的染色（3例中的3例）。筛查研究之外的其他t（1；19）病例显示出相似结果。公共微阵列表达数据库挖掘显示，B淋巴细胞白血病中的BCL6信使核糖核酸表达水平与蛋白表达结果相关。最后，在t（1；19）B淋巴细胞白血病病例中B细胞发育的其他标志物与BCL6表达相关，LMO2和HGAL蛋白分别在9例中的6例（67%）和9例中的8例（89%）中表达。