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Monoclonal antibodies localize the exchangeable GTP-binding site in beta- and not alpha-tubulins.

作者信息

Hesse J, Maruta H, Isenberg G

出版信息

FEBS Lett. 1985 Jan 1;179(1):91-5. doi: 10.1016/0014-5793(85)80198-8.

DOI:10.1016/0014-5793(85)80198-8
PMID:2578113
Abstract

A combination of several methods was used to localize the exchangeable GTP-binding site in the alpha/beta-tubulin heterodimer: direct photoaffinity labeling with [alpha-32P]GTP, specific labeling of alpha- and beta-tubulin by tyrosylation and phosphorylation, respectively, and immunoprecipitation with specific monoclonal antibodies. Direct evidence was obtained that GTP binds exclusively to beta- and not alpha-tubulins.

摘要

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FEBS Lett. 1985 Jan 1;179(1):91-5. doi: 10.1016/0014-5793(85)80198-8.
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体外对单个微管进行紫外线微束照射后，切断的微管末端的不对称行为。
J Cell Biol. 1989 Mar;108(3):931-7. doi: 10.1083/jcb.108.3.931.