Bigbee J W, Calabrese V P, DeVries G H
J Neuroimmunol. 1985 Jan;7(4):221-9. doi: 10.1016/s0165-5728(84)80022-3.
An antiserum was raised to rat central nervous system (CNS) axolemma-enriched fractions (AEF), which showed no cross-reactivity with myelin proteins or liver microsomes yet gave an endpoint titer of 1:51 200 to CNS AEF by the enzyme-linked immunosorbent assay (ELISA). Immunochemical staining of electroblotted proteins from rat CNS and peripheral nervous system (PNS) AEFs separated by gel electrophoresis identified a major reactive band at 38.5 kD. CNS AEF also showed major immunoreactivity at 91 kD (+/- 3 kD) and a broad band from 110 kD to 130 kD. By immunoperoxidase staining the antiserum specifically recognized the axolemma of peripheral nerve and synaptic terminals in the CNS. The significance of the specificity is discussed with respect to anti-synaptosome antisera.
制备了一种针对大鼠中枢神经系统（CNS）富含轴膜组分（AEF）的抗血清，该抗血清与髓磷脂蛋白或肝微粒体无交叉反应，但通过酶联免疫吸附测定（ELISA）对CNS AEF的终点效价为1:51 200。对通过凝胶电泳分离的大鼠CNS和外周神经系统（PNS）AEF的电印迹蛋白进行免疫化学染色，在38.5 kD处鉴定出一条主要反应带。CNS AEF在91 kD（±3 kD）处也显示出主要免疫反应性，以及一条从110 kD到130 kD的宽带。通过免疫过氧化物酶染色，该抗血清特异性识别外周神经的轴膜和CNS中的突触终末。针对抗突触体抗血清讨论了这种特异性的意义。
