通过免疫印迹法鉴定抗中枢神经系统和抗周围神经系统髓磷脂血清中的抗体，采用免疫组织化学法进行表征，并研究其在组织培养中的作用。

Identification of antibodies in anti-CNS and anti-PNS myelin sera by immunoblot, characterization by immunohistochemistry, and their effect in tissue culture.

作者信息

Agrawal H C, Clark H B, Agrawal D, Seil F J, Quarles R H

出版信息

Brain Res. 1984 Jul 30;307(1-2):191-200. doi: 10.1016/0006-8993(84)90473-6.

PMID:6205724

Abstract

Immunoblot analysis of antiserum to rat central nervous system (CNS) myelin revealed antibodies to myelin basic protein (MBP), proteolipid protein (PLP), and numerous high molecular weight proteins. In addition, anti-CNS myelin serum exclusively immunostained 4 basic proteins of rat peripheral nervous system (PNS) myelin. Similarly, anti-PNS myelin sera immunostained many high molecular weight proteins in both CNS and PNS myelin in addition to P0 and 4 basic proteins. Purified MBP and PLP were immunostained by anti-CNS myelin sera and MBP and P0 by anti-PNS myelin sera, indicating that antigenic sites are preserved during protein purification. Immunohistochemical localization with antisera was confined to the myelin sheath except that antisera to CNS myelin also stained oligodendrocytes during the active period of myelination. While anti-CNS myelin sera specifically demyelinated centrally myelinated fibers in culture, none of the anti-PNS myelin sera used here demyelinated organotypic spinal cord-dorsal root ganglion cultures.

摘要

对大鼠中枢神经系统（CNS）髓磷脂抗血清的免疫印迹分析显示，存在针对髓磷脂碱性蛋白（MBP）、蛋白脂蛋白（PLP）以及众多高分子量蛋白的抗体。此外，抗CNS髓磷脂血清仅对大鼠外周神经系统（PNS）髓磷脂的4种碱性蛋白进行免疫染色。同样，抗PNS髓磷脂血清除了对P0和4种碱性蛋白进行免疫染色外，还对CNS和PNS髓磷脂中的许多高分子量蛋白进行免疫染色。纯化的MBP和PLP被抗CNS髓磷脂血清免疫染色，MBP和P0被抗PNS髓磷脂血清免疫染色，这表明抗原位点在蛋白质纯化过程中得以保留。使用抗血清进行免疫组织化学定位仅限于髓鞘，不过抗CNS髓磷脂血清在髓鞘形成活跃期也会对少突胶质细胞进行染色。虽然抗CNS髓磷脂血清在培养中能特异性地使中枢髓鞘化纤维脱髓鞘，但此处使用的抗PNS髓磷脂血清均未使器官型脊髓 - 背根神经节培养物脱髓鞘。