Pawelec G, Schneider E M, Müller C, Wernet P
Eur J Immunol. 1985 Jan;15(1):12-7. doi: 10.1002/eji.1830150104.
Class II major histocompatibility complex determinants restricting recognition of tuberculin antigens (purified protein derivative; PPD) were studied by using monoclonal antibodies (mAb) to block lymphoproliferative responses. Anti-class II mAb were shown to exert inhibitory effects at the level of the antigen-presenting cells, without inducing suppressive lymphocytes or macrophages. Using panels of HLA-typed antigen-presenting cells and nonalloreactive proliferative T cell lines, derived by limiting dilution, restriction elements for PPD responses appeared to correlate with the donor's HLA-DRw6 specificity (one clone), MB1 (one clone), MB3 (one clone), or no established class II (or class I) specificity (three clones). mAb TU22, reacting with nonpolymorphic DC-like determinants, strongly inhibited stimulation of all clones except that restricted by DR antigens, suggesting the DC-like character not only of the MB1- and MB3-associated, but also of the unassigned, restriction elements of these cloned lines. In contrast, stimulation of the DR-restricted line was strongly inhibited by DR/SB-specific mAb which only weakly inhibited the stimulation of clones restricted by DC-like determinants. These results suggest that clonally distributed PPD-reactive proliferative lymphocytes from a single donor may be restricted by at least three different class II determinants (HLA-DR, MB, or a second, novel, DC-related molecule).
通过使用单克隆抗体（mAb）阻断淋巴细胞增殖反应，研究了限制结核菌素抗原（纯化蛋白衍生物；PPD）识别的II类主要组织相容性复合体决定簇。抗II类mAb显示在抗原呈递细胞水平发挥抑制作用，而不诱导抑制性淋巴细胞或巨噬细胞。使用HLA分型的抗原呈递细胞和通过有限稀释获得的非同种异体反应性增殖性T细胞系，PPD反应的限制元件似乎与供体的HLA-DRw6特异性（一个克隆）、MB1（一个克隆）、MB3（一个克隆）相关，或与未确定的II类（或I类）特异性（三个克隆）无关。与非多态性树突状细胞样决定簇反应的mAb TU22强烈抑制除受DR抗原限制的所有克隆的刺激，表明这些克隆系中与MB1和MB3相关的以及未确定的限制元件不仅具有树突状细胞样特征。相反，DR/SB特异性mAb强烈抑制DR限制系的刺激，而对受树突状细胞样决定簇限制的克隆的刺激仅产生微弱抑制。这些结果表明，来自单个供体的克隆分布的PPD反应性增殖淋巴细胞可能受至少三种不同的II类决定簇（HLA-DR、MB或第二种新型的与树突状细胞相关的分子）限制。
