Ogasawara K
Hokkaido Igaku Zasshi. 1984 May;59(3):346-55.
In this study, in vitro immune responses to PPD were analysed using T cell clones and monoclonal antibodies against DR and MB antigens. Effects of monoclonal antibodies on proliferative responses of PBM (peripheral blood mononuclear cell) to PPD (primary response) and on those of PPD-primed PBM to PPD (secondary response) were tested. The results showed that anti-DR monoclonal antibodies, HU-4 and HU-20, inhibited PPD specific PBM proliferative responses through inhibition of antigen presentation with steric hindrance to DR antigens of APC (antigen presenting cell). In contrast, an anti-MB 3 monoclonal antibody, HU-18, did not affect any of the responses tested. Furthermore, three PPD specific T cell clones established were restricted by DR molecules, but not by MB molecules. Thus, it was concluded that DR antigens played an important role in the immune responses to PPD. This result is different from the findings in mice where I-A antigens serve as restriction molecules in PPD specific responses. This difference in restriction molecules between species was discussed.
在本研究中，使用T细胞克隆以及针对DR和MB抗原的单克隆抗体分析了对结核菌素纯蛋白衍生物（PPD）的体外免疫反应。测试了单克隆抗体对外周血单核细胞（PBM）对PPD的增殖反应（初次反应）以及对经PPD致敏的PBM对PPD的增殖反应（二次反应）的影响。结果显示，抗DR单克隆抗体HU-4和HU-20通过空间位阻抑制抗原呈递细胞（APC）的DR抗原，从而抑制PPD特异性PBM增殖反应。相比之下，抗MB 3单克隆抗体HU-18对所测试的任何反应均无影响。此外，所建立的三个PPD特异性T细胞克隆受DR分子限制，而不受MB分子限制。因此，得出结论，DR抗原在对PPD的免疫反应中起重要作用。这一结果与小鼠中的发现不同，在小鼠中I-A抗原在PPD特异性反应中作为限制分子。讨论了不同物种之间限制分子的这种差异。
