Leiden Institute of Chemistry and The Institute for Chemical Immunology, Leiden University, Einsteinweg 55, 2333 CC Leiden (The Netherlands).
Angew Chem Int Ed Engl. 2015 May 4;54(19):5628-31. doi: 10.1002/anie.201500301. Epub 2015 Mar 17.
The activation of CD8(+) T-cells requires the uptake of exogenous polypeptide antigens and proteolytic processing of these antigens to octamer or nonamer peptides, which are loaded on MHC-I complexes and presented to the T-cell. By using an azide as a bioorthogonal protecting group rather than as a ligation handle, masked antigens were generated-antigens that are not recognized by their cognate T-cell unless they are deprotected on the cell using a Staudinger reduction.
CD8(+) T 细胞的激活需要摄取外源性多肽抗原，并对这些抗原进行蛋白水解处理，生成八聚体或九聚体肽，然后将这些肽加载到 MHC-I 复合物上，并呈递给 T 细胞。通过使用叠氮化物作为生物正交保护基团，而不是作为连接手柄，生成了被掩蔽的抗原——除非在细胞上使用施蒂丁格还原将其脱保护，否则这些抗原不会被其同源 T 细胞识别。
