Ratcliffe A, Beauvais P J, Saed-Nejad F
Department of Orthopaedic Surgery, Columbia University, New York, New York.
J Orthop Res. 1994 Jul;12(4):464-73. doi: 10.1002/jor.1100120403.
The levels of proteoglycan aggregate components (link protein, keratan sulfate epitope, and total sulfated glycosaminoglycan) were determined in the synovial fluid lavages of dogs with experimental osteoarthritis or disuse atrophy. A model of experimental osteoarthritis was created by transection of the anterior cruciate ligament of the right knee; studies were carried out 6 and 12 weeks after surgery. Joint disuse was studied at 4 and 8 weeks after initiation of the disuse. Recovery after disuse also was studied in joints that had 3 weeks of remobilization after 4 or 8 weeks of disuse. Synovial fluid lavages from the right knee joints of untreated animals were used as controls. The concentrations of keratan sulfate epitope, sulfated glycosaminoglycan, and link protein in the synovial fluid lavages at 6 and 12 weeks after transection of the anterior cruciate were elevated compared with the control values. Similar analysis of the fluid after disuse showed that the levels of keratan sulfate epitope and sulfated glycosaminoglycan were increased compared with the control levels and the levels after transection. However, the concentration of link protein in the fluid after disuse was not significantly different from the control level. The levels of keratan sulfate epitope and sulfated glycosaminoglycan in the synovial fluid lavages after disuse with recovery were high, but the levels of link protein remained low. The results indicate that the catabolism of proteoglycan aggregates in articular cartilage during early osteoarthritis and disuse is different. The determination of keratan sulfate epitope in synovial fluid lavages appears to provide a relatively general indication of proteoglycan catabolism, whereas increased levels of link protein may be more indicative of cartilage degeneration.
在患有实验性骨关节炎或废用性萎缩的犬类的滑膜液灌洗物中，测定了蛋白聚糖聚集体成分（连接蛋白、硫酸角质素表位和总硫酸化糖胺聚糖）的水平。通过切断右膝前交叉韧带建立实验性骨关节炎模型；在手术后6周和12周进行研究。在废用开始后的4周和8周研究关节废用情况。在废用4周或8周后进行3周重新活动的关节中，也研究了废用后的恢复情况。将未处理动物右膝关节的滑膜液灌洗物用作对照。与对照值相比，前交叉韧带切断后6周和12周滑膜液灌洗物中硫酸角质素表位、硫酸化糖胺聚糖和连接蛋白的浓度升高。对废用后液体的类似分析表明，与对照水平和切断后水平相比，硫酸角质素表位和硫酸化糖胺聚糖的水平升高。然而，废用后液体中连接蛋白的浓度与对照水平无显著差异。废用后恢复的滑膜液灌洗物中硫酸角质素表位和硫酸化糖胺聚糖的水平较高，但连接蛋白的水平仍然较低。结果表明，早期骨关节炎和废用期间关节软骨中蛋白聚糖聚集体的分解代谢是不同的。滑膜液灌洗物中硫酸角质素表位的测定似乎为蛋白聚糖分解代谢提供了一个相对普遍的指标，而连接蛋白水平升高可能更表明软骨退变。
