异地萌发作为一种评估泥炭地兰花阔蕊兰种子活力的方法。

Ex situ germination as a method for seed viability assessment in a peatland orchid, Platanthera blephariglottis.

作者信息

Lemay Marc-André, De Vriendt Laurent, Pellerin Stéphanie, Poulin Monique

机构信息

Université Laval, Pavillon Paul-Comtois, Faculté des sciences de l'agriculture et de l'alimentation, Département de phytologie, 2425 rue de l'Agriculture, Québec, QC, Canada, G1V 0A6 Québec Centre for Biodiversity Science, McGill University, 19 1205 Dr. Penfield Avenue, Montréal, QC, Canada, H3A 1B1.

Institut de recherche en biologie végétale, Jardin botanique de Montréal, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC, Canada, H1X 2B2 Québec Centre for Biodiversity Science, McGill University, 19 1205 Dr. Penfield Avenue, Montréal, QC, Canada, H3A 1B1.

出版信息

Am J Bot. 2015 Mar;102(3):390-5. doi: 10.3732/ajb.1400441. Epub 2015 Mar 1.

DOI:10.3732/ajb.1400441

PMID:25784472

Abstract

UNLABELLED

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PREMISE OF THE STUDY

Assessing seed quality in orchids has been hindered by stringent germination requirements. Seed quality has traditionally been assessed in orchids using in vitro or in situ germination protocols or viability staining. However, these methods are not always well suited for rapid assessment of viability in the context of ecological studies.•

METHODS

The potential of an ex situ protocol for seed viability assessment of orchids in ecological studies was investigated by sowing seeds of Platanthera blephariglottis on Sphagnum moss collected in the orchid's natural habitat. Ex situ germination results were compared with those obtained by viability staining using triphenyltetrazolium chloride (TTC), and the effect of seed testa color on staining and germination results was investigated.•

KEY RESULTS

The ex situ protocol yielded high germination rates, with 66% of the seeds germinating after 9 wk. Depending on the seed testa color class, ex situ germination rates were about 1.4 to 2.5 times higher than viability rates determined using TTC, indicating that the TTC technique underestimated viability compared with the method using ex situ germination. The TTC estimates of viability rates were higher for seeds with dark-colored testae than for pale ones, whereas seed testa color had no effect on germination.•

CONCLUSIONS

Our study showed promising results for the use of ex situ germination as an alternative to previously developed protocols for seed viability assessment of orchids in ecological studies. Staining using TTC might not be well suited for this purpose, since it introduced a bias with respect to seed testa color.

摘要

未标注

研究前提：兰花种子质量评估因严格的萌发要求而受阻。传统上，兰花种子质量评估采用体外或原位萌发方案或活力染色法。然而，在生态研究背景下，这些方法并不总是非常适合快速评估活力。
方法：通过将阔蕊兰种子播种在从兰花自然栖息地采集的泥炭藓上，研究了一种异地方案在生态研究中评估兰花种子活力的潜力。将异地萌发结果与使用氯化三苯基四氮唑（TTC）进行活力染色获得的结果进行比较，并研究种皮颜色对染色和萌发结果的影响。
主要结果：异地方案产生了高萌发率，9周后66%的种子萌发。根据种皮颜色类别，异地萌发率比使用TTC测定的活力率高约1.4至2.5倍，这表明与异地萌发方法相比，TTC技术低估了活力。深色种皮种子的TTC活力率估计值高于浅色种子，而种皮颜色对萌发没有影响。
结论：我们的研究表明，在生态研究中，将异地萌发作为评估兰花种子活力的替代方法具有良好前景。使用TTC染色可能不太适合此目的，因为它在种皮颜色方面引入了偏差。