Imaizumi A, Suzuki Y, Sato H, Sato Y
Vox Sang. 1985;48(1):18-25. doi: 10.1111/j.1423-0410.1985.tb00140.x.
The protective effects of three types of pooled human gamma-globulin preparations (intact: GG, S-sulfonated: GGS, and pepsin-treated: GGP) for intravenous use against experimental aerosol infection of mice with Bordetella pertussis have been evaluated. The gamma-globulin preparations GG and GGS showed significant protective activity whereas GGP did not, as evaluated by survival numbers, body weight gains and suppression of leucocytosis. GG and GGS but not GGP also possessed neutralizing activity against leucocytosis-promoting and histamine-sensitizing activities of pertussis toxin (PT). Evaluation of protective activities of GG, GGS and GGP prepared from rabbit gamma-globulin, highly immunized with PT and not containing any anti-F-HA (filamentous or agglutinin antibodies, demonstrated that anti-PT-GG and anti-PT-GGS but not anti-PT-GGP protected against experimental infection by B. pertussis. These studies showed that the protective activity was correlated with anti-PT titre but that the Fc portion of the gamma-globulin molecule is necessary for actual protection against whooping cough by B. pertussis.
已评估了三种静脉注射用人γ-球蛋白制剂（完整型：GG、S-磺化型：GGS和胃蛋白酶处理型：GGP）对小鼠经气溶胶感染百日咳博德特氏菌的保护作用。通过存活数量、体重增加和白细胞增多抑制情况评估，γ-球蛋白制剂GG和GGS显示出显著的保护活性，而GGP则没有。GG和GGS而非GGP对百日咳毒素（PT）的促白细胞增多和组胺致敏活性也具有中和活性。对用PT高度免疫且不含任何抗F-HA（丝状或凝集素抗体）的兔γ-球蛋白制备的GG、GGS和GGP的保护活性评估表明，抗PT-GG和抗PT-GGS而非抗PT-GGP能保护小鼠免受百日咳博德特氏菌的实验性感染。这些研究表明，保护活性与抗PT滴度相关，但γ-球蛋白分子的Fc部分对于实际抵抗百日咳博德特氏菌引起的百日咳是必要的。
