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通过固态发酵两种接骨木属植物与黑曲霉获得的浆果渣中的总酚含量、抗氧化活性和脂质成分。

Total phenolic contents, antioxidant activities, and lipid fractions from berry pomaces obtained by solid-state fermentation of two Sambucus species with Aspergillus niger.

机构信息

†Faculty of Agriculture, Department of Environmental and Plant Protection, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, Romania.

‡SC Proplanta SRL, CCD-BIODIATECH, Trifoiului 12 G, 400372 Cluj-Napoca, Romania.

出版信息

J Agric Food Chem. 2015 Apr 8;63(13):3489-500. doi: 10.1021/acs.jafc.5b00520. Epub 2015 Mar 26.

Abstract

The aim of this study was to investigate the effect of solid-state fermentation (SSF) by Aspergillus niger on phenolic contents and antioxidant activity in Sambucus nigra L. and Sambucus ebulus L. berry pomaces. The effect of fermentation time on the total fats and major lipid classes (neutral and polar) was also investigated. During the SSF, the extractable phenolics increased with 18.82% for S. ebulus L. and 11.11% for S. nigra L. The levels of antioxidant activity of methanolic extracts were also significantly enhanced. The HPLC-MS analysis indicated that the cyanidin 3-sambubioside-5-glucoside is the major phenolic compound in both fermented Sambucus fruit residues. In the early stages of fungal growth, the extracted oils (with TAGs as major lipid fraction) increased with 12% for S. nigra L. and 10.50% for S. ebulus L. The GC-MS analysis showed that the SSF resulted in a slight increase of the linoleic and oleic acids level.

摘要

本研究旨在探讨黑穗醋栗(Sambucus nigra L.)和接骨木(Sambucus ebulus L.)浆果渣经黑曲霉固态发酵(SSF)后对酚类物质含量和抗氧化活性的影响。还研究了发酵时间对总脂肪和主要脂质类(中性和极性)的影响。在 SSF 过程中,黑穗醋栗和接骨木的可提取酚类物质分别增加了 18.82%和 11.11%。甲醇提取物的抗氧化活性也显著增强。HPLC-MS 分析表明,发酵后的接骨木果实残渣中主要的酚类化合物为矢车菊素 3-桑布双糖苷-5-葡萄糖苷。在真菌生长的早期阶段,黑穗醋栗和接骨木的提取油(以 TAG 为主要脂质部分)分别增加了 12%和 10.50%。GC-MS 分析表明,SSF 导致亚油酸和油酸水平略有增加。

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