Vogel Mathias, Boschke Elke, Bley Thomas, Lenk Felix
Institute of Food Technology and Bioprocess Engineering, Technische Universität Dresden, Dresden, Germany.
Institute of Food Technology and Bioprocess Engineering, Technische Universität Dresden, Dresden, Germany
J Lab Autom. 2015 Aug;20(4):447-56. doi: 10.1177/2211068215576191. Epub 2015 Mar 18.
Due to the size of the required equipment, automated laboratory systems are often unavailable or impractical for use in small- and mid-sized laboratories. However, recent developments in automation engineering provide endless possibilities for incorporating benchtop devices. Here, the authors describe the development of a platform technology to handle sealed culture dishes. The programming is based on the Petri net method and implemented via Codesys V3.5 pbF. The authors developed a system of three independent electrical driven axes capable of handling sealed culture dishes. The device performs two difference processes. First, it automatically obtains an image of every processed culture dish. Second, a server-based image analysis algorithm provides the user with several parameters of the cultivated sample on the culture dish. For demonstration purposes, the authors developed a continuous, systematic, nondestructive, and quantitative method for monitoring the growth of a hairy root culture. New results can be displayed with respect to the previous images. This system is highly accurate, and the results can be used to simulate the growth of biological cultures. The authors believe that the innovative features of this platform can be implemented, for example, in the food industry, clinical environments, and research laboratories.
由于所需设备的尺寸，自动化实验室系统在中小型实验室中往往无法使用或不实用。然而，自动化工程的最新发展为整合台式设备提供了无限可能。在此，作者描述了一种用于处理密封培养皿的平台技术的开发。该编程基于Petri网方法，并通过Codesys V3.5 pbF实现。作者开发了一个由三个独立电动轴组成的系统，能够处理密封培养皿。该设备执行两个不同的过程。首先，它自动获取每个处理过的培养皿的图像。其次，基于服务器的图像分析算法为用户提供培养皿上培养样本的几个参数。为了演示目的，作者开发了一种连续、系统、无损和定量的方法来监测毛状根培养物的生长。新结果可以相对于先前的图像显示。该系统高度准确，其结果可用于模拟生物培养物的生长。作者认为，该平台的创新特性可应用于例如食品工业、临床环境和研究实验室。
