Lee Tae-Hyung, Kim Won-Tae, Ryu Chun Jeih, Jang Young-Joo
a Department of Nanobiomedical Science and BK21 PLUS Global Research Center for Regenerative Medicine, Dankook University, 29 Anseo-Dong, Cheonan 330-714, South Korea.
b Department of Bioscience and Biotechnology, Sejong University, 98 Gunja-Dong, Gwangjin-Gu, Seoul 143-747, South Korea.
Biochem Cell Biol. 2015 Aug;93(4):298-305. doi: 10.1139/bcb-2014-0140. Epub 2015 Feb 26.
Basic fibroblast growth factor (bFGF or FGF-2) is widely used to modulate the proliferation and differentiation of certain cell types. An expression and purification system for recombinant human FGF-2 in Escherichia coli was established for the purpose of securing a continuous supply of this protein. The purified recombinant FGF-2 significantly increased the population of human embryonic stem cells. The optimal concentrations of FGF-2 for cell proliferative induction in various adult stem cells including human dental pulp stem cells, full term human periodontal ligament stem cells, human gingival fibroblasts, mesenchymal stem cells, and osteogenic oseosarcoma were established in a dose-dependent manner. When cells were treated with recombinant FGF-2 for 6 days before osteogenic induction, the mRNA expression of the bone markers was upregulated in cells originated from human dental pulp tissue, indicating that pretreatment with FGF-2 during culture increase stem cell/progenitor population and osteogenic potential.
碱性成纤维细胞生长因子（bFGF或FGF-2）被广泛用于调节某些细胞类型的增殖和分化。为了确保该蛋白的持续供应，建立了一种在大肠杆菌中表达和纯化重组人FGF-2的系统。纯化的重组FGF-2显著增加了人类胚胎干细胞的数量。以剂量依赖的方式确定了FGF-2在包括人牙髓干细胞、足月人牙周膜干细胞、人牙龈成纤维细胞、间充质干细胞和成骨肉瘤细胞在内的各种成体干细胞中诱导细胞增殖的最佳浓度。当成骨诱导前用重组FGF-2处理细胞6天时，源自人牙髓组织的细胞中骨标志物的mRNA表达上调，这表明培养过程中用FGF-2预处理可增加干细胞/祖细胞数量和成骨潜能。
