不使用外源硫醇通过天然化学连接法合成富含半胱氨酸的肽。
Synthesis of cysteine-rich peptides by native chemical ligation without use of exogenous thiols.
作者信息
Tsuda Shugo, Yoshiya Taku, Mochizuki Masayoshi, Nishiuchi Yuji
机构信息
†Peptide Institute, Inc., Ibaraki, Osaka 567-0085, Japan.
‡Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
出版信息
Org Lett. 2015 Apr 3;17(7):1806-9. doi: 10.1021/acs.orglett.5b00624. Epub 2015 Mar 19.
Native chemical ligation (NCL) performed without resorting to the use of thiol additives was demonstrated to be an efficient and effective procedure for synthesizing Cys-rich peptides. This method using tris(2-carboxyethyl)phosphine (TCEP) as a reducing agent facilitates the ligation reaction even at the Thr-Cys or Ile-Cys site and enables one-pot synthesis of Cys-rich peptides throughout NCL and oxidative folding.
不使用硫醇添加剂进行的天然化学连接（NCL）被证明是合成富含半胱氨酸（Cys）肽的一种高效方法。这种使用三（2-羧乙基）膦（TCEP）作为还原剂的方法，即使在苏氨酸-半胱氨酸（Thr-Cys）或异亮氨酸-半胱氨酸（Ile-Cys）位点也能促进连接反应，并能通过NCL和氧化折叠实现富含半胱氨酸肽的一锅法合成。
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