通过分子内S→S酰基转移加速无硫醇添加剂的天然化学连接反应

Acceleration of thiol additive-free native chemical ligation by intramolecular S → S acyl transfer.

作者信息

Schmalisch J, Seitz O

机构信息

Humboldt-Universität zu Berlin, Institut für Chemie, Brook-Taylor-Str. 2, 12489 Berlin, Germany.

出版信息

Chem Commun (Camb). 2015 May 1;51(35):7554-7. doi: 10.1039/c5cc01447f.

DOI:10.1039/c5cc01447f

PMID:25846105

Abstract

Peptide-mercaptopropionylcysteine (MPA-Cys) thioesters show a surprisingly high reactivity in native chemical ligation (NCL) and allow thiol-additive free reactions. This facilitates sequential NCL reactions and ligation-desulfurization reactions in one-pot formats. The synthetic utility is demonstrated by the synthesis of a SH3 domain.

摘要

肽-巯基丙酰半胱氨酸（MPA-Cys）硫酯在天然化学连接（NCL）中表现出惊人的高反应活性，并允许无硫醇添加剂反应。这有利于一锅法进行连续的NCL反应和连接-脱硫反应。通过合成一个SH3结构域证明了其合成效用。