College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi 030006, PR China.
Environ Sci Technol. 2015 Apr 7;49(7):4600-8. doi: 10.1021/es506293t. Epub 2015 Mar 27.
Multiple pollutants, usually at low levels, coexist and may interact in the environment. It is therefore important to analyze the toxicity of mixtures of coexisting chemicals to evaluate the potential ecological risk. Concern regarding the co-occurrence and combined bioeffects of heavy metals and organic insecticides in aquatic settings has existed for many years, but a clear understanding of the interactions between and potential combined toxicity of these chemicals remains elusive. In the present study, the combined effects of the heavy metal nickel (NiSO4) and insect growth regulator buprofezin on the induction of embryo toxicity in zebrafish were assessed. By applying nonlinear regression to the concentration-response data with each of the chemicals using the Hill and Langmuir functions and computing the predictions using the model of concentration addition (CA), we confirmed that NiSO4 and buprofezin acted together to produce synergistic embryotoxicity in zebrafish. Subsequently, we further found that the combination of NiSO4 and buprofezin formed a complex that facilitated the uptake of nickel (Ni) and buprofezin by the embryos. Following this, we clarified that an oxidative mechanism of the complex might underlie the synergistic embryotoxicity of NiSO4 and buprofezin.
多种污染物通常以低水平共存，并可能在环境中相互作用。因此，分析共存化学物质混合物的毒性对于评估潜在的生态风险非常重要。多年来，人们一直关注水生环境中重金属和有机杀虫剂的共存和联合生物效应，但对于这些化学物质之间的相互作用和潜在的联合毒性仍缺乏清晰的认识。本研究评估了重金属镍（NiSO4）和昆虫生长调节剂噻嗪酮对斑马鱼胚胎毒性的诱导的联合效应。通过使用 Hill 和 Langmuir 函数对每种化学物质的浓度-反应数据进行非线性回归，并使用浓度加和（CA）模型计算预测，我们证实 NiSO4 和噻嗪酮共同作用对斑马鱼产生协同胚胎毒性。随后，我们进一步发现 NiSO4 和噻嗪酮形成了一种复合物，促进了镍（Ni）和噻嗪酮被胚胎吸收。在此之后，我们阐明了该复合物的氧化机制可能是 NiSO4 和噻嗪酮协同胚胎毒性的基础。
