Zhong M, Zhao X, Liu Y, Wang Y, Cao K
Acta Virol. 2015 Mar;59(1):49-56. doi: 10.4149/av_2015_01_49.
Cucumber green mottle mosaic virus (CGMMV) is an important and widespread seed-borne virus that infects Cucurbitaceous plants. It is a member of the genus Tobamovirus in the family Virgaviridae with a monopartite (+) ssRNA genome. Here we report the complete genome sequence, construction and testing of the infectious clones of a chb isolate of CGMMV. Full-length CGMMV cDNA was cloned into the vector pUC19. The linearized vector containing full-length cDNA was used as template for in vitro transcription, and the synthesized capped transcript was highly infectious in Chenopodium amaranticolor and cucumber (Cucumis sativus). Inoculated plants showed symptoms typical of CGMMV infection. The infectivity was confirmed by mechanical transmission to new plants, RT-PCR and western blot. Progeny virus derived from infectious transcripts had the same biological and biochemical properties as wild-type virus. To our knowledge, this is the first detailed report of a biologically active transcript from CGMMV.
黄瓜绿斑驳花叶病毒（CGMMV）是一种重要且广泛传播的种传病毒，可感染葫芦科植物。它是烟草花叶病毒属的成员，属于烟草花叶病毒科，具有单分体（+）ssRNA基因组。在此，我们报告了CGMMV一个chb分离株的完整基因组序列、感染性克隆的构建及测试。全长CGMMV cDNA被克隆到载体pUC19中。以含有全长cDNA的线性化载体为模板进行体外转录，合成的加帽转录本在苋色藜和黄瓜（黄瓜属）中具有高度感染性。接种的植物表现出CGMMV感染的典型症状。通过机械接种到新植株、RT-PCR和western印迹证实了其感染性。来自感染性转录本的子代病毒具有与野生型病毒相同的生物学和生化特性。据我们所知，这是关于CGMMV具有生物活性转录本的首份详细报告。
