Li Yongqiang, Deng Congliang, Shang Qiaoxia, Zhao Xiaoli, Liu Xingliang, Zhou Qi
College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
Arch Virol. 2016 Feb;161(2):455-8. doi: 10.1007/s00705-015-2687-5. Epub 2015 Nov 19.
Virus-derived small interfering RNAs (vsiRNAs) of cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, were characterised in cucumber plants by deep sequencing. CGMMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CGMMV-infected cucumber plants. The 5'-terminal base of vsiRNAs was biased towards C/A/U, suggesting that CGMMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were also predicted.
通过深度测序对黄瓜绿斑驳花叶病毒（CGMMV，烟草花叶病毒属成员）的病毒衍生小干扰RNA（vsiRNA）在黄瓜植株中进行了表征。长度为21 - 22个核苷酸的CGMMV vsiRNA占主导地位，这表明在vsiRNA的生物合成过程中可能存在DCL2和DCL4参与的保守机制，以及在CGMMV感染的黄瓜植株中存在共同的RNA沉默途径。vsiRNA的5'末端碱基偏向于C/A/U，这表明CGMMV vsiRNA可能被装载到不同的含AGO的RNA诱导沉默复合体（RISC）中，以干扰宿主植物的基因表达。还预测了一些vsiRNA的可能靶标。
