Matthews R, Scoging A, Rees A D
Immunology. 1985 Jan;54(1):17-23.
Macrophage activating factor (MAF) is produced by antigen-stimulated lymphocytes and activates macrophages for antimicrobial function. The capacity of individual microbial antigens to evoke and regulate this response has been explored using an affinity purified antigen (TB68) of Mycobacterium tuberculosis in combination with T-cell cloning. Four helper/inducer clones are described which responded strongly to this antigen. Three were specific, proliferating only to TB68 antigen and antigenic preparations containing this antigen. However, one of these clones (68.1) did not proliferate to BCG and PPD which contained the TB68 antigen. In addition, another clone, 68.13, also proliferated to other antigenic preparations which did not contain the TB68 antigen. Taken together, these data indicate the presence of several epitopes in the affinity-purified TB68 antigen. All the clones produced MAF, which enhanced H2O2 production in U937 cell lines and conventional macrophages matured from monocytes. Thus, T-cell clones proliferating to a mycobacterial antigen constitutively secrete lymphokines that activate macrophages to antimicrobial immunity.
巨噬细胞激活因子（MAF）由抗原刺激的淋巴细胞产生，并激活巨噬细胞发挥抗菌功能。利用结核分枝杆菌的亲和纯化抗原（TB68）结合T细胞克隆技术，研究了单个微生物抗原引发和调节这种反应的能力。描述了四个对该抗原强烈反应的辅助/诱导克隆。其中三个具有特异性，仅对TB68抗原和含有该抗原的抗原制剂增殖。然而，这些克隆中的一个（68.1）对含有TB68抗原的卡介苗和结核菌素纯蛋白衍生物不增殖。此外，另一个克隆68.13也对不含TB68抗原的其他抗原制剂增殖。综上所述，这些数据表明亲和纯化的TB68抗原中存在多个表位。所有克隆均产生MAF，其增强了U937细胞系和从单核细胞成熟而来的传统巨噬细胞中的过氧化氢生成。因此，对分枝杆菌抗原增殖的T细胞克隆组成性地分泌淋巴因子，激活巨噬细胞产生抗菌免疫。
