Nacy C A, Leonard E J, Meltzer M S
J Immunol. 1981 Jan;126(1):204-7.
Lymphokine-rich culture supernatants of antigen- or mitogen-stimulated spleen cells were fractionated by Sephadex G-200 column chromatography; fractions were assayed for capacity to induce tumoricidal and rickettsiacidal activities in mouse macrophages. Lymphokine activity that activated macrophages for tumor cytotoxicity eluted as a single peak in the 45,000 m.w. region. In contrast, activity for intracellular killing of rickettsiae eluted in 3 distinct regions: 115 to 125,000, 35 to 45,000, and less than 10,000 daltons. This elution pattern was observed with both antigen and mitogen-induced lymphokines. Activity of each of the 3 lymphokine species for induction of rickettsiacidal activity was destroyed by heating at 56 degrees C for 1 hr. Tumor cytotoxicity and rickettsiacidal activity, both effector functions of activated macrophages, were dissociated on the basis of lymphokines regulating these activities.
抗原或丝裂原刺激的脾细胞富含淋巴因子的培养上清液，通过葡聚糖G - 200柱色谱进行分级分离；对各组分诱导小鼠巨噬细胞产生杀肿瘤和杀立克次体活性的能力进行测定。激活巨噬细胞产生肿瘤细胞毒性的淋巴因子活性，在分子量45,000区域以单一峰洗脱。相比之下，细胞内杀灭立克次体的活性在3个不同区域洗脱：115至125,000、35至45,000和小于10,000道尔顿。抗原和丝裂原诱导的淋巴因子均观察到这种洗脱模式。3种淋巴因子中每种诱导杀立克次体活性的能力，在56℃加热1小时后均被破坏。肿瘤细胞毒性和杀立克次体活性，作为激活巨噬细胞的两种效应功能，基于调节这些活性的淋巴因子而分离。
