Verbon A, Hartskeerl R A, Schuitema A, Kolk A H, Young D B, Lathigra R
N.H. Swellengrebel Institute of Tropical Hygeine, Royal Tropical Institute, Amsterdam, The Netherlands.
J Bacteriol. 1992 Feb;174(4):1352-9. doi: 10.1128/jb.174.4.1352-1359.1992.
Eight monoclonal antibodies (MAbs) directed against the 14,000-molecular-weight (14K) antigen of Mycobacterium tuberculosis reacted specifically with mycobacteria of the M. tuberculosis complex. The nucleotide sequence of the gene encoding the 14K antigen was determined by using recombinant DNA clones isolated from lambda gt11 and cosmid libraries of the M. tuberculosis genome. The DNA sequence of the 14K protein gene coded for a polypeptide of 144 amino acids with a calculated molecular mass of 16,277 Da. The 14K antigen has a marked homology with proteins belonging to the alpha-crystallin family of low-molecular-weight heat shock proteins, which includes the 18K antigen of M. leprae. The eight MAbs recognized at least four distinct epitopes localized within the following three regions of the 14K protein: amino acids 10 to 92 (MAbs F67-8 and F67-16), amino acids 41 to 92 (F159-1 and F159-11), and amino acids 41 to 144 (F23-41, F24-2, F23-49, and TB68).
8种针对结核分枝杆菌14000分子量（14K）抗原的单克隆抗体（MAb）与结核分枝杆菌复合群的分枝杆菌发生特异性反应。通过使用从结核分枝杆菌基因组的λgt11和黏粒文库中分离的重组DNA克隆，确定了编码14K抗原的基因的核苷酸序列。14K蛋白基因的DNA序列编码了一个由144个氨基酸组成的多肽，计算分子量为16277道尔顿。14K抗原与属于低分子量热休克蛋白α-晶状体蛋白家族的蛋白质具有显著同源性，该家族包括麻风分枝杆菌的18K抗原。这8种单克隆抗体识别了位于14K蛋白以下三个区域内的至少4个不同表位：氨基酸10至92（单克隆抗体F67-8和F67-16）、氨基酸41至92（F159-1和F159-11）以及氨基酸41至144（F23-41、F24-2、F23-49和TB68）。
