Burtin P, Chavanel G, Andre J
Int J Cancer. 1985 Mar 15;35(3):307-14. doi: 10.1002/ijc.2910350305.
We studied the plasmin system with specific antisera to plasminogen, its 2 activators (urokinase-type and tissue-type) and the 2 plasmin inhibitors, alpha 2 anti-plasmin and alpha 2 macroglobulin on sections of 34 human colonic tumors by immunofluorescence. Anti-plasminogen serum showed a clear-cut reactivity at the surface of tumor cells, as it stained the contour of tumor glandular structures, foci, and isolated tumor cells. Intratumoral deposits and necrotic areas were stained as well, often strongly. Localization of plasminogen was quite different from that of fibrinogen, which was found only in peritumoral stroma, and never on tumor cells. Traces of both types of plasminogen activator were found, mainly on invasive tumor cells for urokinase type and on large tumor foci for tissue type. Images were weak and inconstant. Large amounts of both plasmin inhibitors were characterized in tumor stroma. Alpha-2 anti-plasmin was also found in intratumoral deposits and necrotic areas. It seems likely that plasminogen exudes from blood capillaries (since anti-plasminogen serum often stained the whole capillary wall), diffuses in the stroma and binds to tumor cells. Once formed, plasmin is likely to play a role in the invasion of surrounding tissues by tumor cells, in the dissociation of tumor cells from tumor glands and in the production of necrosis inside tumor areas.
我们用针对纤溶酶原、其2种激活剂（尿激酶型和组织型）以及2种纤溶酶抑制剂α2抗纤溶酶和α2巨球蛋白的特异性抗血清，通过免疫荧光法研究了34例人类结肠肿瘤切片中的纤溶酶系统。抗纤溶酶原血清在肿瘤细胞表面显示出明确的反应性，它能染出肿瘤腺管结构、病灶和单个肿瘤细胞的轮廓。瘤内沉积物和坏死区域也常被强烈染色。纤溶酶原的定位与纤维蛋白原的定位截然不同，纤维蛋白原仅在肿瘤周围基质中发现，从未在肿瘤细胞上发现。两种类型的纤溶酶原激活剂均有微量发现，尿激酶型主要存在于侵袭性肿瘤细胞上，组织型主要存在于大的肿瘤病灶上。图像较弱且不稳定。在肿瘤基质中发现了大量的两种纤溶酶抑制剂。α2抗纤溶酶也存在于瘤内沉积物和坏死区域。纤溶酶原似乎从毛细血管渗出（因为抗纤溶酶原血清常使整个毛细血管壁染色），在基质中扩散并与肿瘤细胞结合。一旦形成，纤溶酶可能在肿瘤细胞侵袭周围组织、肿瘤细胞与肿瘤腺管解离以及肿瘤区域内坏死的产生中发挥作用。
