Lebar R, Lees M B
J Neuroimmunol. 1985 Apr;8(1):43-55. doi: 10.1016/s0165-5728(85)80046-1.
The unidentified antigen M2, which is the target of demyelinating antibodies in sera from guinea pigs immunized with brain tissue, was studied using a dot immunobinding assay. This method is particularly appropriate for studying the immunologic properties of insoluble brain components such as myelin, glycolipids and myelin proteins. M2 was found to be a component of central nervous system myelin and not peripheral myelin. It appeared at the time of myelination, was not cerebroside or GM1 ganglioside and did not cross-react with the major myelin proteins, i.e. proteolipid or basic protein. These observations confirm and extend previous studies using a complement fixation assay for M2. The antigen was shown to be partially solubilized by Brij 35 and this detergent can provide a starting point for attempts at purification.
