Barre Léna, Brasseur Emilie, Doux Camille, Lombard Bertrand, Besse Nathalie Gnanou
French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
French Agency for Food, Environmental and Occupational Health Safety, Laboratory for Food Safety, ANSES, 23 avenue du Général de Gaulle, 94706 Maisons Alfort cedex, France.
Food Microbiol. 2015 Jun;48:171-7. doi: 10.1016/j.fm.2014.10.012. Epub 2014 Dec 24.
For the enumeration of Listeria monocytogenes (L. monocytogenes) in food, a sensitive enumeration method has been recently developed. This method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate L. monocytogenes. An evaluation of this method was performed with several categories of foods naturally contaminated with L. monocytogenes. The results obtained with this technique were compared with those obtained from the modified reference EN ISO 11290-2 method for the enumeration of L. monocytogenes in food, and are found to provide more precise results. In most cases, the filtration method enabled to examine a greater quantity of food thus greatly improving the sensitivity of the enumeration. However, it was hardly applicable to some food categories because of filtration problems and background microbiota interference.
为了对食品中的单核细胞增生李斯特菌进行计数，最近开发了一种灵敏的计数方法。该方法基于对食品悬浮液进行膜过滤，然后将滤膜转移到选择性培养基上以计数单核细胞增生李斯特菌。用几类天然被单核细胞增生李斯特菌污染的食品对该方法进行了评估。将该技术获得的结果与用于食品中单核细胞增生李斯特菌计数的改良参考EN ISO 11290-2方法获得的结果进行比较，发现该方法能提供更精确的结果。在大多数情况下，过滤方法能够检测更多的食品，从而大大提高了计数的灵敏度。然而，由于过滤问题和背景微生物群的干扰，该方法几乎不适用于某些食品类别。
