Eng A M, Jin Y T, Matsuoka L Y, Grgurich C V, Robinson J, Armin A
J Cutan Pathol. 1985 Feb;12(1):46-54. doi: 10.1111/j.1600-0560.1985.tb00429.x.
Twenty-one specimens of Verruca vulgaris were used for an investigation to define what portion of the papillomatous structures seen in H & E stain will correspond to the areas of positive staining for papilloma virus antigen utilizing the peroxidase-antiperoxidase immunostaining technique. Vacuolar spaces filled with thready material and large ovoid parakeratotic cells seen in H & E stain stained positively for papilloma virus antigen. In the granular layer, viral antigens stained heavily, the nuclei of cells showing koilocytosis. Both round and irregularly shaped kerathohyaline granules did not stain positively for viral antigen stain. Eight of the 21 specimens showed marked positivity for papilloma virus antigen. Three other cases were positive in only focal sites. Of the 8 immunostained-positive selected cases used for ultrastructural studies, only 2 showed the viral particles. The study suggests that the PAP immunostaining is a more sensitive and yet less cumbersome method than electronmicroscopy for demonstration of papilloma virus.
使用21个寻常疣标本进行一项研究，以确定利用过氧化物酶-抗过氧化物酶免疫染色技术，在苏木精和伊红（H&E）染色中所见的乳头状结构的哪一部分将对应于乳头瘤病毒抗原的阳性染色区域。在H&E染色中可见充满细丝状物质的空泡间隙和大的卵圆形不全角化细胞，它们对乳头瘤病毒抗原呈阳性染色。在颗粒层，病毒抗原染色很深，显示有凹空细胞的细胞核。圆形和不规则形的透明角质颗粒对病毒抗原染色均未呈阳性。21个标本中有8个对乳头瘤病毒抗原呈明显阳性。另外3例仅在局灶部位呈阳性。在用于超微结构研究的8例免疫染色阳性的选定病例中，只有2例显示有病毒颗粒。该研究表明，对于乳头瘤病毒的显示，过氧化物酶-抗过氧化物酶免疫染色是一种比电子显微镜更敏感且更简便的方法。
