Lucia H L, LiVolsi V A, Lowell D M
Am J Clin Pathol. 1984 Nov;82(5):589-93. doi: 10.1093/ajcp/82.5.589.
In order to study the location of cells infected with human papilloma virus in paraffin-embedded tissues, the authors developed the following stain. Tissue sections were digested overnight with 0.01% deoxyribonuclease and then stained using the Feulgen stain with a light green counterstain. Cellular DNA was degraded, but viral DNA was not. Thus infected cells retained magneta nuclei, while uninfected cells stained a homogenous green. The stain was positive in 5/5 plantar warts, 7/8 verruca vulgaris, 2/2 laryngeal papilloma, and 18/20 condylomata accuminata. As a comparison, the indirect immunoperoxidase method on the same tissues stained 5/5 plantar warts, 6/8 verruca vulgaris, 0/2 laryngeal warts, and 2/20 condylomata accuminata. The presence of virus was confirmed by electron microscopic examination on one of the sections. Molluscum contageosum infected tissue also stained positively, but tissues infected with cytomegalovirus and herpes simplex virus did not. The only nonviral positive staining occurred with smears of sperm. The reasons for these results are discussed.
为了研究人乳头瘤病毒感染细胞在石蜡包埋组织中的定位，作者开发了以下染色方法。组织切片用0.01%的脱氧核糖核酸酶消化过夜，然后用福尔根染色并以淡绿色复染。细胞DNA被降解，但病毒DNA未被降解。因此，感染的细胞保留紫红色细胞核，而未感染的细胞则染成均匀的绿色。该染色法在5/5例跖疣、7/8例寻常疣、2/2例喉乳头瘤和18/20例尖锐湿疣中呈阳性。作为对照，对相同组织采用间接免疫过氧化物酶法染色，结果在5/5例跖疣、6/8例寻常疣、0/2例喉疣和2/20例尖锐湿疣中呈阳性。通过对其中一张切片进行电子显微镜检查证实了病毒的存在。传染性软疣感染组织也呈阳性染色，但巨细胞病毒和单纯疱疹病毒感染的组织则未呈阳性。唯一非病毒阳性染色出现在精子涂片上。文中讨论了这些结果的原因。
