一种小非编码RNA制剂的定量与质量控制

Quantification and quality control of a small non-coding RNA preparation.

作者信息

Marchand Virginie, Branlant Christiane

机构信息

Université de Lorraine, CNRS UMR 7365 IMoPA, Vandoeuvre-lès-Nancy, France,

出版信息

Methods Mol Biol. 2015;1296:17-28. doi: 10.1007/978-1-4939-2547-6_3.

DOI:10.1007/978-1-4939-2547-6_3

PMID:25791587

Abstract

Recent advances in high-throughput sequencing have shed some new light on the diversity of small non-coding RNA (sncRNA) classes and their crucial role in gene regulation and diseases. RNA quantification and control of RNA integrity are two key steps in sncRNA profiling. In this chapter, we will describe different gold standard methods used to achieve both purposes before the use of the RNAs in downstream applications.

摘要

高通量测序技术的最新进展为小非编码RNA（sncRNA）类别的多样性及其在基因调控和疾病中的关键作用带来了一些新的认识。RNA定量和RNA完整性控制是sncRNA分析中的两个关键步骤。在本章中，我们将描述在将RNA用于下游应用之前用于实现这两个目的的不同金标准方法。