小非编码RNA的乙醇沉淀

Alcoholic precipitation of small non-coding RNAs.

作者信息

Clerget Guillaume, Bourguignon-Igel Valérie, Rederstorff Mathieu

机构信息

Université de Lorraine, CNRS UMR 7365 IMoPA, Biopôle, 9 avenue de la Forêt de Haye, Vandoeuvre-lès-Nancy, 54506, France.

出版信息

Methods Mol Biol. 2015;1296:11-6. doi: 10.1007/978-1-4939-2547-6_2.

DOI:10.1007/978-1-4939-2547-6_2

PMID:25791586

Abstract

Alcoholic precipitation is a critical step to recover RNA of high purity. This chapter describes the principles of alcoholic precipitation as well as a standard, basic protocol with key advices to observe, but numerous variations on the theme are discussed. Indeed, several important parameters, such as the choice of salt, alcohol, or carrier, have to be considered to improve the efficiency of precipitation and the yield of RNA recovery.

摘要

乙醇沉淀是获得高纯度RNA的关键步骤。本章介绍了乙醇沉淀的原理以及一个标准的基本方案，并给出了关键的注意事项，同时还讨论了该方案的多种变体。实际上，为了提高沉淀效率和RNA回收率，必须考虑几个重要参数，如盐、乙醇或载体的选择。