Tang Ying, Shen Yafeng, Huang Libin, Lv Gaojian, Lei Changhai, Fan Xiaoyan, Lin Fangxing, Zhang Yuxia, Wu Lihui, Yang Yongji
Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China.
Department of Science & Research, Second Military Medical University, Shanghai 200433, China.
Environ Toxicol Pharmacol. 2015 Mar;39(2):871-8. doi: 10.1016/j.etap.2015.02.003. Epub 2015 Feb 16.
Gold nanoparticles, which have unique physicochemical characteristics, are being used for an increasingly wide range of applications in biomedical research. In this study, gold nanorods (width of 25 nm, length of 52 nm) were found to be internalized by A549 cells and were primarily localized in the lysosomes and membranous vesicles. The integrity of the membranes of A549 cells exposed to gold nanorods for 4h was damaged, as indicated by laser scanning confocal microscopy (LSCM). Increased lactate dehydrogenase (LDH) leakage and decreased cell viability further indicated the concentration-dependent cytotoxicity of the gold nanorods to the A549 cells. Reactive oxygen species (ROS) production was induced in the A549 cells by the gold nanorods, and this effect was positively correlated with the concentration of the gold nanorods. The results of this study indicated that exposure to gold nanorods caused dose-dependent cytotoxicity in A549 cells and that oxidative stress may be the main factor causing cytotoxicity.
具有独特物理化学特性的金纳米颗粒在生物医学研究中的应用范围越来越广泛。在本研究中，发现金纳米棒（宽度为25纳米，长度为52纳米）被A549细胞内化，并主要定位于溶酶体和膜泡中。激光扫描共聚焦显微镜（LSCM）显示，暴露于金纳米棒4小时的A549细胞膜的完整性受到破坏。乳酸脱氢酶（LDH）泄漏增加和细胞活力下降进一步表明金纳米棒对A549细胞具有浓度依赖性细胞毒性。金纳米棒在A549细胞中诱导了活性氧（ROS）的产生，并且这种效应与金纳米棒的浓度呈正相关。本研究结果表明，暴露于金纳米棒会导致A549细胞产生剂量依赖性细胞毒性，氧化应激可能是导致细胞毒性的主要因素。
