铂浪通可维持脓毒症大鼠血脑屏障的完整性。

Platonin preserves blood-brain barrier integrity in septic rats.

作者信息

Yeh Chia-Tse, Kao Ming-Chang, Chen Cay-Huyen, Huang Chun-Jen

机构信息

Department of Family Medicine, Taipei Tzu Chi Hospital, Taipei, Taiwan.

Department of Anesthesiology, Taipei Tzu Chi Hospital, Taipei, Taiwan; School of Medicine, Tzu Chi University, Hualien, Taiwan.

出版信息

Acta Anaesthesiol Taiwan. 2015 Mar;53(1):12-5. doi: 10.1016/j.aat.2015.02.001. Epub 2015 Mar 17.

DOI:10.1016/j.aat.2015.02.001

PMID:25791838

Abstract

OBJECTIVES

Platonin possesses potent anti-inflammatory and antioxidative capacities. Because systemic inflammation and oxidative stress are crucial in mediating sepsis-induced blood-brain barrier (BBB) integrity loss, this study elucidated the effects of platonin on preserving BBB integrity in septic rats.

METHODS

A total of 72 adult male rats (200-250 g) were randomized to receive cecal ligation and puncture (CLP), CLP plus platonin, sham operation, or sham operation plus platonin (n = 18 in each group). Systemic inflammation and oxidation levels and BBB integrity in the surviving rats were determined after 24-hour monitoring.

RESULTS

Plasma levels of interleukin-6 (IL-6) and malondialdehyde (MDA)-markers of systemic inflammation and oxidation-and the grading of Evans blue staining of the brains, BBB permeability to Evans blue dye, and brain edema levels-markers of BBB integrity-in rats that received CLP were significantly higher than rats that received sham operation (all p < 0.001). By contrast, the plasma levels of IL-6 (p < 0.001) and MDA (p < 0.001), and the grading of Evans blue staining (p = 0.015), BBB permeability to Evans blue dye (p = 0.043), and brain edema levels (p = 0.034) in rats that received CLP plus platonin were significantly lower than rats that received CLP. Experimental data further revealed that the concentration of tight junction protein claudin-5, a major structural component of BBB, in rats that received CLP was significantly lower than rats that received CLP plus platonin (p = 0.023).

CONCLUSION

Platonin could attenuate sepsis-induced BBB integrity loss in rats.

摘要

目的

普拉托宁具有强大的抗炎和抗氧化能力。由于全身炎症和氧化应激在介导脓毒症诱导的血脑屏障（BBB）完整性丧失中起关键作用，本研究阐明了普拉托宁对脓毒症大鼠血脑屏障完整性的保护作用。

方法

将72只成年雄性大鼠（200 - 250克）随机分为接受盲肠结扎穿孔术（CLP）、CLP加普拉托宁、假手术或假手术加普拉托宁组（每组n = 18）。24小时监测后，测定存活大鼠的全身炎症和氧化水平以及血脑屏障完整性。

结果

接受CLP的大鼠血浆中白细胞介素 - 6（IL - 6）和丙二醛（MDA）水平（全身炎症和氧化的标志物）以及脑伊文思蓝染色分级、血脑屏障对伊文思蓝染料的通透性和脑水肿水平（血脑屏障完整性的标志物）均显著高于接受假手术的大鼠（所有p < 0.001）。相比之下，接受CLP加普拉托宁的大鼠血浆中IL - 6（p < 0.001）和MDA（p < 0.001）水平，以及伊文思蓝染色分级（p = 0.015）、血脑屏障对伊文思蓝染料的通透性（p = 0.043）和脑水肿水平（p = 0.034）均显著低于接受CLP的大鼠。实验数据进一步显示，接受CLP的大鼠血脑屏障主要结构成分紧密连接蛋白claudin - 5的浓度显著低于接受CLP加普拉托宁的大鼠（p = 0.023）。