Aksoy Ayse Nur, Toker Aysun, Celık Muhammet, Aksoy Mehmet, Halıcı Zekai, Aksoy Hulya
Department of Obsterics and Gynecology, Nenehatun Hospital, Erzurum, Turkey.
Department of Biochemistry, Meram Faculty of Medicine, Konya University, Konya, Turkey.
Indian J Pharmacol. 2014 Nov-Dec;46(6):622-6. doi: 10.4103/0253-7613.144922.
To explore the protective effect of progesterone on inflammation and oxidative stress in a rat model of sepsis created by cecal ligation and puncture (CLP).
Rats were randomly divided into 4 groups: Overiectomy group (OVX), sham operated (control), sepsis (CLP) group and progesterone-treated CLP group (CLP+ progesterone). The rats in CLP+ progesterone group received intraperitoneal progesterone (2 mg/kg). Cardiac blood samples were obtained for the measurement levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α). Tissue samples, including liver, kidney and uterus of rats were prepared to determine activities of myeloperoxidase (MPO), glutathione peroxidase (GPx) and levels of malondialdehyde (MDA).
Increased serum IL-6 and TNF-α levels were found in the CLP group in comparison with the control group (P = 0.01, P = 0.02; respectively). In CLP+ progesterone group, mean MDA concentration of kidney tissue was significantly lower than in CLP group (P = 0.003). Liver MDA concentration of the CLP+ progesterone group was not significantly different from that of the control group. While there were no significant differences among groups regarding liver MPO; in the CLP group, MPO activity in kidney (P = 0.02) and uterine tissues (P = 0.03) were found to be significantly higher compared to the control group. In CLP+ progesterone group, mean MPO activities of all tissues were not different than those of control group. The uterine tissue GPx activity in the CLP+ progesterone group was not statistically significantly different from control group.
We suggest that progesterone ameliorates sepsis syndrome by reduction of the inflammatory cytokines IL-6 and TNF-α, and by restoration of antioxidant enzyme activities in some tissues.
探讨孕酮对盲肠结扎穿刺（CLP）所致脓毒症大鼠模型炎症和氧化应激的保护作用。
将大鼠随机分为4组：去卵巢组（OVX）、假手术（对照）组、脓毒症（CLP）组和孕酮治疗CLP组（CLP + 孕酮）。CLP + 孕酮组大鼠腹腔注射孕酮（2 mg/kg）。采集心脏血样以测定白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平。制备大鼠肝脏、肾脏和子宫等组织样本，以测定髓过氧化物酶（MPO）、谷胱甘肽过氧化物酶（GPx）活性及丙二醛（MDA）水平。
与对照组相比，CLP组血清IL-6和TNF-α水平升高（分别为P = 0.01，P = 0.02）。CLP + 孕酮组肾脏组织平均MDA浓度显著低于CLP组（P = 0.003）。CLP + 孕酮组肝脏MDA浓度与对照组无显著差异。各组肝脏MPO无显著差异；与对照组相比，CLP组肾脏（P = 0.02）和子宫组织（P = 0.03）中MPO活性显著升高。CLP + 孕酮组所有组织的平均MPO活性与对照组无差异。CLP + 孕酮组子宫组织GPx活性与对照组无统计学显著差异。
我们认为，孕酮通过降低炎症细胞因子IL-6和TNF-α以及恢复某些组织中的抗氧化酶活性来改善脓毒症综合征。
