2',3'-环核苷酸3'-磷酸二酯酶和髓磷脂碱性蛋白在颤抖小鼠和正常小鼠中枢神经系统中的免疫组织化学定位

Immunohistochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase and myelin basic protein in the central nervous system of the jimpy and the normal mouse.

作者信息

Mikoshiba K, Kohsaka S, Hayakawa T, Takamatsu K, Tsukada Y

出版信息

J Neurochem. 1985 Mar;44(3):686-91. doi: 10.1111/j.1471-4159.1985.tb12869.x.

DOI:10.1111/j.1471-4159.1985.tb12869.x

Abstract

We describe the immunohistochemical localization of 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) and myelin basic protein (MBP) in CNS of the jimpy mutant mouse which is characterized by dys- and demyelination. In controls, the CNPase and MBP were localized exclusively in white matter in the CNS. The jimpy mutant mice were severely affected: A very weak reaction was observed in the white matter. Very few CNPase- and MBP-positive myelin sheaths were observed, and some degradation products were also observed after reaction with antisera against both CNPase and MBP. The immunohistochemical reaction in the jimpy mice showed a similar localization in both CNPase and MBP.

摘要

我们描述了2',3'-环核苷酸3'-磷酸二酯酶（CNPase）和髓鞘碱性蛋白（MBP）在以脱髓鞘和髓鞘发育异常为特征的jimpy突变小鼠中枢神经系统中的免疫组织化学定位。在对照组中，CNPase和MBP仅定位于中枢神经系统的白质中。jimpy突变小鼠受到严重影响：在白质中观察到非常微弱的反应。观察到极少的CNPase和MBP阳性髓鞘，并且在用抗CNPase和MBP的抗血清反应后也观察到一些降解产物。jimpy小鼠中的免疫组织化学反应在CNPase和MBP中显示出相似的定位。