聚乙二醇化脂质体反式茴香脑对乳腺癌细胞系MCF-7和T47D的抗癌活性。
Anti-cancer activity of pegylated liposomal trans-anethole on breast cancer cell lines MCF-7 and T47D.
作者信息
Shahbazian Shahedeh, Akbarzadeh Azim, Torabi Sepideh, Omidi Mansour
机构信息
Department of Agricultural Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran,
出版信息
Biotechnol Lett. 2015 Jul;37(7):1355-9. doi: 10.1007/s10529-015-1813-5. Epub 2015 Mar 20.
OBJECTIVE
To examine the role of liposomes for the encapsulation of drugs and their suitability for chemotherapy of breast cancer.
RESULTS
Pegylated liposomal trans-anethole nanoparticles were synthesized through a reverse-phase evaporation technique. Nanoparticles were characterized in terms of mean diameter, size distribution, zeta potential, encapsulation and drug loading efficiency, drug release pattern and cytotoxicity effects. Size and zeta potential of pegylated nanoliposomal drug and blank pegylated nanoliposomal were 257 nm and -28 mV; 35.7 nm and -21 mV, respectively. Encapsulation and drug loading efficiency were 78 ± 2.5 and 2.3 ± 4.1 %, respectively. There was a 57 % release of trans-anethole from pegylated liposomal nanoparticles in 48 h. Compared to free drug, toxicological studies indicated around 9- and 8-fold cytotoxicity effect against MCF-7 and T47D cell lines respectively.
CONCLUSIONS
PEG-liposomes provided a high stability and slow release of trans-anethole in two cancer cell lines.
目的
研究脂质体在药物包封中的作用及其对乳腺癌化疗的适用性。
结果
通过反相蒸发技术合成了聚乙二醇化反式茴香脑纳米脂质体。对纳米颗粒进行了平均直径、粒径分布、zeta电位、包封率、载药效率、药物释放模式和细胞毒性作用等方面的表征。聚乙二醇化纳米脂质体药物和空白聚乙二醇化纳米脂质体的大小和zeta电位分别为257 nm和-28 mV；35.7 nm和-21 mV。包封率和载药效率分别为78±2.5%和2.3±4.1%。在48小时内，反式茴香脑从聚乙二醇化脂质体纳米颗粒中的释放率为57%。毒理学研究表明，与游离药物相比，对MCF-7和T47D细胞系的细胞毒性作用分别约高9倍和8倍。
结论
聚乙二醇脂质体在两种癌细胞系中提供了反式茴香脑的高稳定性和缓释性。
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