Ebrahimifar Meysam, Nili-Ahmadabadi Amir, Akbarzadeh Azim, Shahemabadi Hasan Ebrahimi, Hasanzadegan Majid, Moradi-Sardareh Hemen, Madadizadeh Hassan, Rezaee-Diyan Jalal
Department of Toxicology, Faculty of Pharmacy, Islamic Azad University, Shahreza Branch, Shahreza, Iran.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Hamadan University of Medical Sciences, P.O. Box 8678-3-65178, Hamadan, Iran.
Indian J Clin Biochem. 2017 Jun;32(2):230-234. doi: 10.1007/s12291-016-0596-3. Epub 2016 Aug 2.
Carboplatin is a chemotherapeutic agent used against various malignancies such as ovarian carcinoma. The aim of this study is to improve the therapeutic efficacy of carboplatin using pegylated liposomal nanocarriers. Nanoparticles were synthesized using thin film hydration technique and characterized for shape morphology, particle size, zeta potential and drug-release properties. In the next step, A2780S and A2780CP ovarian cancer cell lines were used to determine the efficacy of nanodrug by MTT assay. The particle size and zeta potential of nanodrug were measured 244.3 ± 19.6 nm and -22.9 ± 1.7 mV, respectively. High encapsulation capacity (78.6 ± 3.7 %) confirmed the efficiency of technique. The cytotoxicity results also showed that nanodrug compared to free drug improve the efficacy of carboplatin against both A2780S ( < 0.01) and A2780CP ( < 0.05) cell lines. In conclusion, the findings of our study suggested pegylated liposomal nanocarriers are proper for carboplatin delivery to ovarian cancer cell lines A2780S and A2780CP.
卡铂是一种用于治疗多种恶性肿瘤（如卵巢癌）的化疗药物。本研究的目的是使用聚乙二醇化脂质体纳米载体提高卡铂的治疗效果。采用薄膜水化技术合成纳米颗粒，并对其形状形态、粒径、zeta电位和药物释放特性进行表征。下一步，使用A2780S和A2780CP卵巢癌细胞系通过MTT法测定纳米药物的疗效。纳米药物的粒径和zeta电位分别测定为244.3±19.6nm和-22.9±1.7mV。高包封率（78.6±3.7%）证实了该技术的有效性。细胞毒性结果还表明，与游离药物相比，纳米药物提高了卡铂对A2780S（<0.01）和A2780CP（<0.05）细胞系的疗效。总之，我们的研究结果表明，聚乙二醇化脂质体纳米载体适合将卡铂递送至卵巢癌细胞系A2780S和A2780CP。
